Změnit instituci

Winter School of Advanced Fluorescence Microscopy

WHEN: February 16 - 22, 2015
WHERE: University Campus Bohunice

  • Are you interested in deciphering the function and structure of protein complexes?
  • Would you like to study essential interactions of biomolecules in live cells?
  • Do you want to learn from experienced research experts?

Practically taught methods

  1. Light sheet fluorescence microscopy (LSFM) to observe 3D development and biomolecular mobility inside living biological objects
  2. Fluorescence resonance energy transfer (FRET) to detect molecular proximity, applied to nuclear proteins with various modalities (spectral based and acceptor photobleaching) implemented on confocal microscope
  3. Fluorescence correlation spectroscopy (FCS) to measure molecular mobility and binding, applied to transcription factors diffusing in the nucleus and binding to chromatin within the context of regulatory complexes
  4. Fluorescence recovery after photobleaching (FRAP) to study the mobility of proteins in living cells
  5. Total internal reflection fluorescence microscopy (TIRF) to measure of protein and enzyme interaction dynamics and mechanisms

 

If you find the course attractive, please register HERE.

Deadline for your application: 15 January 2015.

More information can be found HERE.

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