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We study processes governing genome stability and chromosome segregation. Our research aims to decipher the molecular mechanisms that stabilize and protect chromosome ends, called telomeres, from being perceived by the cell as DNA damage. We also investigate the regulatory pathways that define meiosis, the cell division necessary for sexual reproduction and the generation of haploid gametes.
Telomeres form the ends of eukaryotic chromosomes and are important for the complete replication of linear genomes and for chromosome stability. We found that the opposite ends of a chromosome adopt different end protective structures, the formation of which is dictated by the mode of DNA replication. Furthermore, we discovered a fundamentally novel mechanism of chromosome end protection that relies on the evolutionary conserved DNA repair complex Ku. In our current research we aim to understand how Ku mediates protection of these telomeres without triggering a DNA repair reaction. In our meiotic research we aim to delineate pathways involved in remodelling of the cell cycle machinery to enable the meiotic mode of chromosome segregation. We have identified Arabidopsis meiotic cyclins and revealed their contribution to diverse meiotic processes. Furthermore, we have discovered a genetic module, consisting of SMG7 and TDM1 that inhibits meiotic CDKs and facilitates the transition from meiosis to mitosis. Our current work is directed towards a molecular understanding of the SMG7/TDM1 function and characterization of additional genes involved in meiotic progression.
seznam / vizitky
Supervisor: Mgr. Karel Říha, Ph.D.
Analyzing various aspects of germ-line differentiation.
Keywords: plant germ-line
Supervisor: Mgr. Karel Říha, Ph.D.
Plant germ-line forms during late stages of plant growth within developing floral organs. We use genetic, cell biology and biochemical tools delineate molecular processes that govern formation of haploid gametophytes from diploid precursors. We are particularly interested in mechanisms that regulate meiosis. We have designed a genetic screen to identify novel genes involved meiotic progression in Arabidopsis thaliana. The PhD project deals with functional characterization of the genes discovered in the screen and development of technologies for in depth analysis of plant germ-line differentiation.
Keywords: plant germ-line
29. ledna 2018 9:46
LECTURE: Dr. Ondrej Hovorka: Models of magnetic nanoparticles for biomedical applications MONDAY, 5. 2. 2018 Seminar room C2.11, from …
25. ledna 2018 18:21
WHEN: 30. 01. 2018 WHERE: CEITEC BUT, Purkynova 123, large meeting room SPEAKER: Dr Andriy Marko TALK: Advances in PELDOR…