Lékařská genomika - Šárka Pospíšilová

Research areas

• Introduction of high-throughput analyses into medical research and diagnostics, development of new diagnostic techniques
• Identification and analysis of novel diagnostic and prognostic markers and therapeutical targets

Main objectives

• Introduction of high-throughput analyses of human genomes (whole genome/exom sequencing, transcriptome profiling, microRNA detection, SNP analysis, etc.). Utilization of these technologies in medicine and development of diagnostic tests based on high-throughput methods
• Mutational analyses of human cells in relation to cancer, neuromuscular, neurodegenerative, metabolic and skin disorders; the detection of novel prognostic markers

Content of research

Application of genomic approaches in cancer research and diagnostics

The transformation of normal to malignant cells may be caused by many different mechanisms that share a single common feature – the alteration of genetic information and subsequent disruption of cellular regulatory mechanisms, which thus lead to uncontrolled proliferation. Some of these genetic alterations have already been described and are routinely analysed in oncological diagnostics, e.g. TP53, ATM or BRCA gene mutations or specific translocations occurring in leukemias, lymphomas and other tumours. The importance of many other genomic aberrations found in tumours and their influence on the malignant potential of transformed cells should be analysed as well as the impact of individual genetic variants on tumour behaviour. Novel technologies including high-resolution SNP microarrays and high-throughput genome sequencing (massive parallel sequencing) provide fast and complex analysis of the human genome. These methods will be used to characterise genetic information of the patient‘s malignant and non-malignant cells to reveal the mechanisms of cellular transformation.

The expected outputs of this work package are:

1) the identification of recurrent genomic alterations in hematological and other malignancies, which could be used in cancer diagnostics and as a potential therapeutic target,

2) the analysis of the influence of the host genome on disease progression,

3) the characterisation of the regulatory pathways disrupted in tumour cells. The outcome of these studies is expected to be used as an initial point for focused research as well as for direct use in diagnostics of haematological and oncological malignancies.

Technologies used: massive parallel DNA sequencing (next-generation high-throughput sequencing), SNP mapping (high resolution SNP microarrays), gene expression profiling (microarrays, QRT-PCR), FACS, bioinformatic processing. 

 

Brochure about the FP7 project "Next Generation Sequencing platform for targeted Personalized Therapy of Leukemia" to download can be found HERE.

seznam / vizitky

VYBRANÉ PUBLIKACE

2017

• BENCUROVA, P; BALOUN, J; MUSILOVA, K; RADOVA, L; TICHY, B; PAIL, M; ZEMAN, M; BRICHTOVA, E; HERMANOVA, M; POSPISILOVA, S; MRAZ, M; BRAZDIL, M, 2017:MicroRNA and mesial temporal lobe epilepsy with hippocampal sclerosis: Whole miRNome profiling of human hippocampus. EPILEPSIA 58 (10), p. 1782 - 1793.
• BOČÁNEK, O; ŠEDO, O; PEKÁR, S; ZDRÁHAL, Z, 2017:Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS. TOXICON 133 , p. 18 - 25.
• BYSTRY, V.; REIGL, T; KREJCI, A; DEMKO, M; HANAKOVA, B; GRIONI, A; KNECHT, H; SCHLITT, M.; DREGER, P; SELLNER, L;HERRMANN, D; PINGEON, M; BOUDJOGHRA, M; RIJNTJES, J; POTT, C; LANGERAK, A W; GROENEN P. J; DAVI, F; BRÜGGEMANN, M; DARZENTAS, N, 2017:ARResT/Interrogate: an interactive immunoprofiler for IG/TR NGS data. 33 (3), p. 435 - 437.
• FIALA, T; LUDVIKOVA, L; HEGER, D; SVEC, J; SLANINA, T; VETRAKOVA, L; BABIAK, M; NECAS, M; KULHANEK, P; KLAN, P; SINDELAR, V, 2017:Bambusuril as a One-Electron Donor for Photoinduced Electron Transfer to Methyl Viologen in Mixed Crystals. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139 (7), p. 2597 - 2603.
• GRODECKA, L; KOVACOVA, T; KRAMAREK, M; SENECA, S; STOUFFS, K; DE LAET, C; MAJER, F; KRSJAKOVA, T; HUJOVA, P; HRNCIROVA, K; SOUCEK, P; LISSENS, W; BURATTI, E; FREIBERGER, T, 2017:Detailed molecular characterization of a novel IDS exonic mutation associated with multiple pseudoexon activation. JOURNAL OF MOLECULAR MEDICINE-JMM 95 (3), p. 299 - 309.
• KANTOROVA, B; MALCIKOVA, J; BRAZDILOVA, K; BORSKY, M; PLEVOVA, K; SMARDOVA, J; RADOVA, L; TOM, N; TRBUSEK, M; DIVISKOVA, E; FRANCOVA, HS; NAVRKALOVA, V; DOUBEK, M; BRYCHTOVA, Y; MAYER, J; POSPISILOVA, S, 2017:Single cell analysis revealed a coexistence of NOTCH1 and TP53 mutations within the same cancer cells in chronic lymphocytic leukaemia patients. BRITISH JOURNAL OF HAEMATOLOGY 178 (6), p. 979 - 982.
• KOTASKOVA, J; PAVLOVA, S; PLEVOVA, K; MALCIKOVA, J; STEHLIKOVA, O; POPPOVA, L; KOCKOVA, H; DOUBEK, M; BRYJA, V; POSPISILOVA, S, 2017:TREATMENT WITH BCR INHIBITORS INCREASES ROR1 EXPRESSION IN CLL CELLS. HAEMATOLOGICA 102 , p. 414 - 414.
• LEDEREROVA, A; KOZLOVA, V; VAKULOVA, V; DOUBEK, M; MAYER, J; POSPISILOVA, S; SMIDA, M, 2017:B CELLS RESISTANT TO CD20 MONOCLONAL ANTIBODIES DISPLAY SPECIFIC ALTERATIONS IN GENE EXPRESSION PROFILE. HAEMATOLOGICA 102 , p. 709 - 709.
• PAL, K; BYSTRY, V; REIGL, T; DEMKO, M; KREJCI, A; TOULOUMENIDOU, T; STALIKA, E; TICHY, B; GHIA, P; STAMATOPOULOS, K; POSPISILOVA, S; MALCIKOVA, J; DARZENTAS, N, 2017:GLASS: assisted and standardized assessment of gene variations from Sanger sequence trace data. BIOINFORMATICS 33 (23), p. 3802 - 3804.
• PAVLASOVA, G; BORSKY, M; SVOBODOVA, V; MUSILOVA, K; CERNA, K; SEDA, V; OSICKOVA, J; BRYCHTOVA, Y; DOUBEK, M; POSPISILOVA, S; MAYER, J; MRAZ, M, 2017:BCR SIGNALLING PROFICIENT CHRONIC LYMPHOCYTIC LEUKAEMIA B CELLS ARE PRONE TO RITUXIMAB MEDIATED ELIMINATION IN VIVO. HAEMATOLOGICA 102 , p. 412 - 412.
• PAVLASOVA, G; MUSILOVA, K; SEDA, V; CERNA, K; VOJACKOVA, E; SVOBODOVA, V; MRAZ, M, 2017:The identification of combinatorial therapeutic approaches with BCR inhibitors in B cell malignancies. MOLECULAR CANCER THERAPEUTICS 16 (10)
• PLEVOVA, K; MALCIKOVA, J; PAVLOVA, S; KOTASKOVA, J; POPPOVA, L; SMARDOVA, J; DIVISKOVA, E; DURECHOVA, K; OLTOVA, A; BRYCHTOVA, Y; PANOVSKA, A; DOUBEK, M; POSPISILOVA, S, 2017:BIALLELIC TP53 GENE MUTATIONS DUE TO COPY-NEUTRAL LOSS OF HETEROZYGOSITY AND MONOALLELIC MUTATIONS IN ABSENCE OF 17P DELETION OCCUR IN CLL WITH COMPARABLE FREQUENCY. HAEMATOLOGICA 102 , p. 65 - 66.
• ROSENQUIST, R; GHIA, P; HADZIDIMITRIOU, A; SUTTON, L A; AGATHANGELIDIS, A; BALIAKAS, P; DARZENTAS, N; GIUDICELLI, V; LEFRANC M. P; LANGERAK, A W; BELESSI, C; DAVI F; STAMATOPOULOS, K, 2017:Immunoglobulin gene sequence analysis in chronic lymphocytic leukemia: Updated eric recommendations. LEUKEMIA
• VARDI, A; VLACHONIKOLA, E; KARYPIDOU, M; STALIKA, E; BIKOS, V; GEMENETZI, K; MARAMIS, C; SIORENTA, A; ANAGNOSTOPOULOS, A; POSPISILOVA, S; MAGLAVERAS, N; CHOUVARDA, I; STAMATOPOULOS, K; HADZIDIMITRIOU, A, 2017:Restrictions in the T-cell repertoire of chronic lymphocytic leukemia: high-throughput immunoprofiling supports selection by shared antigenic elements. LEUKEMIA 31 (7), p. 1555 - 1561.
• YOUNG, E; NOERENBERG, D; MANSOURI, L; LJUNGSTROM, V; FRICK, M; SUTTON, LA; BLAKEMORE, SJ; GALAN-SOUSA, J; PLEVOVA, K; BALIAKAS, P; ROSSI, D; CLIFFORD, R; ROOS-WEIL, D; NAVRKALOVA, V; DORKEN, B; SCHMITT, CA; SMEDBY, KE; JULIUSSON, G; GIACOPELLI, B; BLACHLY, JS; BELESSI, C; PANAGIOTIDIS, P; CHIORAZZI, N; DAVI, F; LANGERAK, AW; OSCIER, D; SCHUH, A; GAIDANO, G; GHIA, P; XU, W; FAN, L; BERNARD, OA; NGUYEN-KHAC, F; RASSENTI, L; LI, J; KIPPS, TJ; STAMATOPOULOS, K; POSPISILOVA, S; ZENZ, T; OAKES, CC; STREFFORD, JC; ROSENQUIST, R; DAMM, F, 2017:EGR2 mutations define a new clinically aggressive subgroup of chronic lymphocytic leukemia. LEUKEMIA 31 (7), p. 1547 - 1554.

2016

• BALIAKAS, P; MATTSSON, M; HADZIDIMITRIOU, A; MINGA, E; AGATHANGELIDIS, A; SUTTON, LA; SCARFO, L; DAVIS, Z; YAN, XJ; PLEVOVA, K; SANDBERG, Y; VOJDEMAN, FJ; TZENOU, T; CHU, CC; VERONEZE, S; MANSOURI, L; SMEDBY, KE; GIUDICELLI, V; NGUYEN-KHAC, F; PANAGIOTIDIS, P; JULIUSSON, G; ANAGNOSTOPOULOS, A; LEFRANC, MP; TRENTIN, L; CATHERWOOD, M; MONTILLO, M; NIEMANN, CU; LANGERAK, AW; POSPISILOVA, S; STAVROYIANNI, N; CHIORAZZI, N; OSCIER, D; JELINEK, DF; SHANAFELT, T; DARZENTAS, N; BELESSI, C; DAVI, F; GHIA, P; ROSENQUIST, R; STAMATOPOULOS, K, 2016:NO IMPROVEMENT IN LONG-TERM OVERALL SURVIVAL AFTER THE INTRODUCTION OF CHEMO(IMMUNO)THERAPY FOR CHRONIC LYMPHOCYTIC LEUKEMIA PATIENTS BELONGING TO STEREOTYPED SUBSET #2. HAEMATOLOGICA 101 , p. 231 - 231.
• BALIAKAS, P; PUIGGROS, A; XOCHELLI, A; SUTTON, LA; NGUYEN-KHAC, F; GARDINER, A; PLEVOVA, K; MINGA, E; HADZIDIMITRIOU, A; WALEWSKA, R; MCCARTHY, H; ORTEGA, M; COLLADO, R; GONZALEZ, T; GRANADA, I; LUNO, E; KOTASKOVA, J; MOYSIADIS, T; DAVIS, Z; STAVROYIANNI, N; ANAGNOSTOPOULOS, A; STREFFORD, JC; POSPISILOVA, S; DAVI, F; ATHANASIADOU, A; ROSENQUIST, R; OSCIER, D; ESPINET, B; STAMATOPOULOS, K, 2016:Additional trisomies amongst patients with chronic lymphocytic leukemia carrying trisomy 12: the accompanying chromosome makes a difference. HAEMATOLOGICA 101 (7), p. 299 - 302.
• BEZDICEK, M; LENGEROVA, M; RICNA, D; WEINBERGEROVA, B; KOCMANOVA, I; VOLFOVA, P; DRGONA, L; POCZOVA, M; MAYER, J; RACIL, Z, 2016:Rapid detection of fungal pathogens in bronchoalveolar lavage samples using panfungal PCR combined with high resolution melting analysis. MEDICAL MYCOLOGY 54 (7), p. 714 - 724.
• BIKOS, V; KARYPIDOU, M; STALIKA, E; BALIAKAS, P; XOCHELLI, A; SUTTON, LA; PAPADOPOULOS, G; AGATHANGELIDIS, A; PAPADOPOULOU, E; DAVIS, Z; ALGARA, P; KANELLIS, G; TRAVERSE-GLEHEN, A; MOLLEJO, M; ANAGNOSTOPOULOS, A; PONZONI, M; GONZALEZ, D; POSPISILOVA, S; MATUTES, E; PIRIS, MA; PAPADAKI, T; GHIA, P; ROSENQUIST, R; OSCIER, D; DARZENTAS, N; TZOVARAS, D; BELESSI, C; HADZIDIMITRIOU, A; STAMATOPOULOS, K, 2016:An Immunogenetic Signature of Ongoing Antigen Interactions in Splenic Marginal Zone Lymphoma Expressing IGHV1-2*04 Receptors. CLINICAL CANCER RESEARCH 22 (8), p. 2032 - 2040.
• BRANNA, P; CERNOCHOVA, J; ROUCHAL, M; KULHANEK, P; BABINSKY, M; MAREK, R; NECAS, M; KURITKA, I; VICHA, R, 2016:Cooperative Binding of Cucurbit[n]urils and beta-Cyclodextrin to Heteroditopic Imidazolium-Based Guests. JOURNAL OF ORGANIC CHEMISTRY 81 (20), p. 9595 - 9604.
• BRITANOVA, OV; SHUGAY, M; MERZLYAK, EM; STAROVEROV, DB; PUTINTSEVA, EV; TURCHANINOVA, MA; MAMEDOV, IZ; POGORELYY, MV; BOLOTIN, DA; IZRAELSON, M; DAVYDOV, AN; EGOROV, ES; KASATSKAYA, SA; REBRIKOV, DV; LUKYANOV, S; CHUDAKOV, DM, 2016:Dynamics of Individual T Cell Repertoires: From Cord Blood to Centenarians. JOURNAL OF IMMUNOLOGY 196 (12), p. 5005 - 5013.
• BRONSON, PG; CHANG, D; BHANGALE, T; SELDIN, MF; ORTMANNL, W; FERREIRA, RC; URCELAY, E; PEREIRA, LF; MARTIN, J; PLEBANI, A; LOUGARIS, V; FRIMAN, V; FREIBERGER, T; LITZMAN, J; THON, V; PAN-HAMMARSTROM, Q; HAMMARSTROM, L; GRAHAM, RR; BEHRENS, TW, 2016:Common variants at PVT1, ATG13-AMBRA1, AHI1 and CLEC16A are associated with selective IgA deficiency. NATURE GENETICS 48 (11), p. 1425 - 1429.
• BUCKOVA, H; NOSKOVA, H; BORSKA, R; REBLOVA, K; PINKOVA, B; ZAPLETALOVA, E; KOPECKOVA, L; HORKY, O; NEMECKOVA, J; GAILLYOVA, R; NAGY, Z; VESELY, K; HERMANOVA, M; STEHLIKOVA, K; FAJKUSOVA, L, 2016:Autosomal recessive congenital ichthyoses in the Czech Republic. BRITISH JOURNAL OF DERMATOLOGY 174 (2), p. 405 - 407.
• CORTES, JE; SAGLIO, G; KANTARJIAN, HM; BACCARANI, M; MAYER, J; BOQUE, C; SHAH, NP; CHUAH, C; CASANOVA, L; BRADLEY-GARELIK, B; MANOS, G; HOCHHAUS, A, 2016:Final 5-Year Study Results of DASISION: The Dasatinib Versus Imatinib Study in Treatment-Naive Chronic Myeloid Leukemia Patients Trial. JOURNAL OF CLINICAL ONCOLOGY 34 (20), p. 2333 - U54.
• CULEN, M; BORSKY, M; NEMETHOVA, V; RAZGA, F; SMEJKAL, J; JURCEK, T; DVORAKOVA, D; ZACKOVA, D; WEINBERGEROVA, B; SEMERAD, L; SADOVNIK, I; EISENWORT, G; HERRMANN, H; VALENT, P; MAYER, J; RACIL, Z, 2016:Quantitative assessment of the CD26+leukemic stem cell compartment in chronic myeloid leukemia: Patient-subgroups, prognostic impact, and technical aspects. ONCOTARGET 7 (22), p. 33016 - 33024.
• GREIL, R; OBRTLIKOVA, P; SMOLEJ, L; KOZAK, T; STEURER, M; ANDEL, J; BURGSTALLER, S; MIKUSKOVA, E; GERCHEVA, L; NOSSLINGER, T; PAPAJIK, T; LADICKA, M; GIRSCHIKOFSKY, M; HRUBISKO, M; JAGER, U; FRIDRIK, M; PECHERSTORFER, M; KRALIKOVA, E; BURCOVEANU, C; SPASOV, E; PETZER, A; MIHAYLOV, G; RAYNOV, J; OEXLE, H; ZABERNIGG, A; FLOCHOVA, E; PALASTHY, S; STEHLIKOVA, O; DOUBEK, M; ALTENHOFER, P; PLEYER, L; MELCHARDT, T; KLINGLER, A; MAYER, J; EGLE, A, 2016:Rituximab maintenance versus observation alone in patients with chronic lymphocytic leukaemia who respond to first-line or second-line rituximab-containing chemoimmunotherapy: final results of the AGMT CLL-8a Mabtenance randomised trial. LANCET HAEMATOLOGY 3 (7), p. E317 - E329.
• GRIONI, A; FAZIO, G; REIGL, T; RIGAMONTI, S; BYSTRY, V; SONGIA, S; BIONDI, A; DARZENTAS, N; CAZZANIGA, G, 2016:A PLATFORM FOR DETECTION OF FUSION GENES IN ALL FROM TARGET CAPTURE NEXT- GENERATION SEQUENCING OF DNA AND RNA. HAEMATOLOGICA 101 , p. 26 - 27.
• HERNANDEZ-SANCHEZ, M; RADOVA, L; KOTASKOVA, J; TAMBORERO, D; RODRIGUEZ, AE; PLEVOVA, K; ABAIGAR, M; BIKOS, V; BENITO, R; TAKACOVA, S; QUIJADA, M; MARTIN, AA; ALCOCEBA, M; DE COCA, AG; DOUBEK, M; GONZALEZ, M; LOPEZ-BIGAS, N; POSPISILOVA, S; HERNANDEZ-RIVAS, JM, 2016:Analysis of Clonal Evolution in Chronic Lymphocytic Leukemia from Inactive to Symptomatic Disease Prior Treatment Using Whole-Exome Sequencing. BLOOD 128 (22)
• HO, AD; SCHETELIG, J; BOCHTLER, T; SCHAICH, M; SCHAFER-ECKART, K; HANEL, M; ROSLER, W; EINSELE, H; KAUFMANN, M; SERVE, H; BERDE, WE; STELLJES, M; MAYER, J; REICHLE, A; BALDUS, CD; SCHMITZ, N; KRAMER, M; ROLLIG, C; BORNHAUSER, M; THIEDE, C; EHNINGER, G, 2016:Allogeneic Stem Cell Transplantation Improves Survival in Patients with Acute Myeloid Leukemia Characterized by a High Allelic Ratio of Mutant FLT3-ITD. BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION 22 (3), p. 462 - 469.
• JANOVSKA, P; POPPOVA, L; PLEVOVA, K; PLESINGEROVA, H; BEHAL, M; KAUCKA, M; OVESNA, P; HLOZKOVA, M; BORSKY, M; STEHLIKOVA, O; BRYCHTOVA, Y; DOUBEK, M; MACHALOVA, M; BASKAR, S; KOZUBIK, A; POSPISILOVA, S; PAVLOVA, S; BRYJA, V, 2016:Autocrine Signaling by Wnt-5a Deregulates Chemotaxis of Leukemic Cells and Predicts Clinical Outcome in Chronic Lymphocytic Leukemia. CLINICAL CANCER RESEARCH 22 (2), p. 459 - 469.
• JASKOVA, Z; MALCIKOVA, J; SMARDOVA, J; BRYCHTOVA, Y; DOUBEK, M; TRBUSEK, M, 2016:PRO-APOPTOTIC EFFECTS OF PRIMA-1MET CORRELATE WITH NOXA GENE INDUCTION IN CHRONIC LYMPHOCYTIC LEUKEMIA CELLS. HAEMATOLOGICA 101 , p. 226 - 226.
• KOPECKOVA, L; BUCKOVA, H; KYROVA, J; GAILLYOVA, R; NEMECKOVA, J; JERABKOVA, B; VESELY, K; STEHLIKOVA, K; FAJKUSOVA, L, 2016:Ten years of DNA diagnostics of epidermolysis bullosa in the Czech Republic. BRITISH JOURNAL OF DERMATOLOGY 174 (6), p. 1388 - 1391.
• KOPPARAPU, PK; BHOI, S; MANSOURI, L; ARABANIAN, LS; PLEVOVA, K; POSPISILOVA, S; WASIK, AM; CROCI, GA; SANDER, B; PAULLI, M; ROSENQUIST, R; KANDURI, M, 2016:Epigenetic silencing of miR-26A1 in chronic lymphocytic leukemia and mantle cell lymphoma: Impact on EZH2 expression. EPIGENETICS 11 (5), p. 335 - 343.
• KOTASKOVA, J; PAVLOVA, S; GREIF, I; STEHLIKOVA, O; PLEVOVA, K; JANOVSKA, P; BRYCHTOVA, Y; DOUBEK, M; POSPISILOVA, S; BRYJA, V, 2016:ROR1-based immunomagnetic protocol allows efficient separation of CLL and healthy B cells. BRITISH JOURNAL OF HAEMATOLOGY 175 (2), p. 339 - 342.
• KRYUKOV, F; NEMEC, P; RADOVA, L; KRYUKOVA, E; OKUBOTE, S; MINARIK, J; STEFANIKOVA, Z; POUR, L; HAJEK, R, 2016:Centrosome associated genes pattern for risk sub-stratification in multiple myeloma. JOURNAL OF TRANSLATIONAL MEDICINE 14
• KUBESOVA, B; PAVLOVA, S; MALCIKOVA, J; KABATHOVA, J; RADOVA, L; TOM, N; TICHY, B; PLEVOVA, K; KANTOROVA, B; FIEDOROVA, K; KISSOVA, J; GISSLINGER, B; GISSLINGER, H; MAYER, J; KRALOVICS, R; POSPISILOVA, S; DOUBEK, M, 2016:Low-Burden TP53 Mutations Occur in Chronic Phase of Myeloproliferative Neoplasms Regardless of Hydroxyurea Administration, Disease Type, and JAK2 Status. BLOOD 128 (22)
• MELAZZINI, F; PALOMBO, F; BALDUINI, A; DE ROCCO, D; MARCONI, C; NORIS, P; GNAN, C; PIPPUCCI, T; BOZZI, V; FALESCHINI, M; BAROZZI, S; DOUBEK, M; DI BUDUO, CA; KOZUBIK, KS; RADOVA, L; LOFFREDO, G; POSPISILOVA, S; ALFANO, C; SERI, M; BALDUINI, CL; PECCI, A; SAVOIA, A, 2016:Clinical and pathogenic features of ETV6-related thrombocytopenia with predisposition to acute lymphoblastic leukemia. HAEMATOLOGICA 101 (11), p. 1333 - 1342.
• MIKULASEK, K; JARON, KS; KULHANEK, P; BITTOVA, M; HAVLIS, J, 2016:Sequence-dependent separation of trinucleotides by ion-interaction reversed-phase liquid chromatography A structure-retention study assisted by soft-modelling and molecular dynamics. JOURNAL OF CHROMATOGRAPHY A 1469 , p. 88 - 95.
• MLCOCHOVA, H; MACHACKOVA, T; RABIEN, A; RADOVA, L; FABIAN, P; ILIEV, R; SLABA, K; POPRACH, A; KILIC, E; STANIK, M; REDOVA-LOJOVA, M; SVOBODA, M; DOLEZEL, J; VYZULA, R; JUNG, K; SLABY, O, 2016:Epithelial-mesenchymal transition-associated microRNA/mRNA signature is linked to metastasis and prognosis in clear-cell renal cell carcinoma. SCIENTIFIC REPORTS 6
• NAVRKALOVA, V; YOUNG, E; BALIAKAS, P; RADOVA, L; SUTTON, LA; PLEVOVA, K; MANSOURI, L; LJUNGSTROM, V; NTOUFA, S; DAVIS, Z; JULIUSSON, G; SMEDBY, KE; BELESSI, C; PANAGIOTIDIS, P; TOULOUMENIDOU, T; DAVI, F; LANGERAK, AW; GHIA, P; STREFFORD, JC; OSCIER, D; MAYER, J; STAMATOPOULOS, K; POSPISILOVA, S; ROSENQUIST, R; TRBUSEK, M, 2016:ATM mutations in major stereotyped subsets of chronic lymphocytic leukemia: enrichment in subset #2 is associated with markedly short telomeres. HAEMATOLOGICA 101 (9)
• PAVLASOVA, G; BORSKY, M; SEDA, V; CERNA, K; OSICKOVA, J; DOUBEK, M; MAYER, J; CALOGERO, R; POSPISILOVA, S; DAVIDS, M; BROWN, J; MRAZ, M, 2016:IBRUTINIB INHIBITS CD20 UP-REGULATION ON CLL B CELLS MEDIATED BY THE CXCR4/SDF-1 AXIS. HAEMATOLOGICA 101 , p. 47 - 47.
• PAVLASOVA, G; BORSKY, M; SEDA, V; CERNA, K; OSICKOVA, J; DOUBEK, M; MAYER, J; CALOGERO, R; TRBUSEK, M; POSPISILOVA, S; DAVIDS, MS; KIPPS, TJ; BROWN, JR; MRAZ, M, 2016:Ibrutinib inhibits CD20 upregulation on CLL B cells mediated by the CXCR4/SDF-1 axis. BLOOD 128 (12), p. 1609 - 1613.
• POPPOVA, L; JANOVSKA, P; PLEVOVA, K; RADOVA, L; PLESINGEROVA, H; BORSKY, M; KOTASKOVA, J; KANTOROVA, B; HLOZKOVA, M; FIGULOVA, J; BRYCHTOVA, Y; DOUBEK, M; KOZUBIK, A; POSPISILOVA, S; PAVLOVA, S; BRYJA, V, 2016:DECREASED EXPRESSION OF WNT3, A CANONICAL WNT PATHWAY LIGAND, IS FREQUENT IN CHRONIC LYMPHOCYTIC LEUKEMIA PROGRESSION AND IDENTIFIES PATIENTS WITH SHORT TREATMENT-FREE SURVIVAL IN MUTATED IGHV SUBSET. HAEMATOLOGICA 101 , p. 423 - 424.
• POPPOVA, L; JANOVSKA, P; PLEVOVA, K; RADOVA, L; PLESINGEROVA, H; BORSKY, M; KOTASKOVA, J; KANTOROVA, B; HLOZKOVA, M; FIGULOVA, J; BRYCHTOVA, Y; MACHALOVA, M; URIK, M; DOUBEK, M; KOZUBIK, A; POSPISILOVA, S; PAVLOVA, S; BRYJA, V, 2016:Decreased WNT3 expression in chronic lymphocytic leukaemia is a hallmark of disease progression and identifies patients with worse prognosis in the subgroup with mutated IGHV. BRITISH JOURNAL OF HAEMATOLOGY 175 (5), p. 851 - 859.
• POSPISILOVA, S; SUTTON, LA; MALCIKOVA, J; TAUSCH, E; ROSSI, D; MONTSERRAT, E; MORENO, C; STAMATOPOULOS, K; GAIDANO, G; ROSENQUIST, R; GHIA, P, 2016:Innovation In the prognostication of chronic lymphocytic leukemia: how far beyond TP53 gene analysis can we go?. HAEMATOLOGICA 101 (3), p. 263 - 265.
• RAWSTRON, AC; FAZI, C; AGATHANGELIDIS, A; VILLAMOR, N; LETESTU, R; NOMDEDEU, J; PALACIO, C; STEHLIKOVA, O; KREUZER, KA; LIPTROT, S; O'BRIEN, D; DE TUTE, RM; MARINOV, I; HAUWEL, M; SPACEK, M; DOBBER, J; KATER, AP; GAMBELL, P; SOOSAPILLA, A; LOZANSKI, G; BRACHTL, G; LIN, K; BOYSEN, J; HANSON, C; JORGENSEN, JL; STETLER-STEVENSON, M; YUAN, C; BROOME, HE; RASSENTI, L; CRAIG, F; DELGADO, J; MORENO, C; BOSCH, F; EGLE, A; DOUBEK, M; POSPISILOVA, S; MULLIGAN, S; WESTERMAN, D; SANDERS, CM; EMERSON, R; ROBINS, HS; KIRSCH, I; SHANAFELT, T; PETTITT, A; KIPPS, TJ; WIERDA, WG; CYMBALISTA, F; HALLEK, M; HILLMEN, P; MONTSERRAT, E; GHIA, P, 2016:A complementary role of multiparameter flow cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study. LEUKEMIA 30 (4), p. 929 - 936.
• SANTOS, RD; GIDDING, SS; HEGELE, RA; CUCHEL, MA; BARTER, PJ; WATTS, GF; BAUM, SJ; CATAPANO, AL; CHAPMAN, MJ; DEFESCHE, JC; FOLCO, E; FREIBERGER, T; GENEST, J; HOVINGH, GK; HARADA-SHIBA, M; HUMPHRIES, SE; JACKSON, AS; MATA, P; MORIARTY, PM; RAAL, FJ; AL-RASADI, K; RAY, KK; REINER, Z; SIJBRANDS, EJG; YAMASHITA, S, 2016:Defining severe familial hypercholesterolaemia and the implications for clinical management: a consensus statement from the International Atherosclerosis Society Severe Familial Hypercholesterolemia Panel. LANCET DIABETES & ENDOCRINOLOGY 4 (10), p. 850 - 861.
• SEDLARIKOVA, L; GROMESOVA, B; KUBACZKOVA, V; RADOVA, L; ALMASI, M; PENKA, M; ADAM, Z; POUR, L; KREJCI, M; SEVCIKOVA, S; HAJEK, R, 2016:LONG NON-CODING RNAS IN MULTIPLE MYELOMA. HAEMATOLOGICA 101 , p. 518 - 518.
• SEDO, O; KORAN, M; JAKESOVA, M; MIKULIKOVA, R; BOHAC, M; ZDRAHAL, Z, 2016:Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry. FOOD CHEMISTRY 206 , p. 124 - 130.
• SHYAMSUNDER, P; ESNER, M; BARVALIA, M; WU, YJ; LOJA, T; BOON, HB; LLEONART, ME; VERMA, RS; KREJCI, L; LYAKHOVICH, A, 2016:Impaired mitophagy in Fanconi anemia is dependent on mitochondrial fission. ONCOTARGET 7 (36), p. 58065 - 58074.
• SCHWARZOVA, L; HORINEK, A; HUBACEK, J; VRABLIK, M; FREIBERGER, T; TICHY, L; VAVERKOVA, H; CESKA, R, 2016:EFFECT OF APOE GENOTYPE ON LDL CHOLESTEROL LEVELS IN FH AND FDB PATIENTS: IS THERE SEX-SPECIFICALLY PROTECTIVE GENOTYPE?. ATHEROSCLEROSIS 252 , p. E40 - E40.
• SLANINOVA, V; KRAFCIKOVA, M; PEREZ-GOMEZ, R; STEFFAL, P; TRANTIREK, L; BRAY, SJ; KREJCI, A, 2016:Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle. OPEN BIOLOGY 6 (2)
• SMIDA, M; DE LA CRUZ, FF; KERZENDORFER, C; URAS, IZ; MAIR, B; MAZOUZI, A; SUCHANKOVA, T; KONOPKA, T; KATZ, AM; PAZ, K; NAGY-BOJARSZKY, K; MUELLNER, MK; BAGO-HORVATH, Z; HAURA, EB; LOIZOU, JI; NIJMAN, SMB, 2016:MEK inhibitors block growth of lung tumours with mutations in ataxia-telangiectasia mutated. NATURE COMMUNICATIONS 7
• SMIDA, M; KOZLOVA, V; VAKULOVA, V; LEDEREROVA, A; DOUBEK, M; MAYER, J; POSPISILOVA, S, 2016:Cellular Mechanisms Regulating CD20 As a Target of Monoclonal Antibody Therapy in B-Lymphoid Malignancies. BLOOD 128 (22)
• SPONER, JE; SPONER, J; NOVAKOVA, O; BRABEC, V; SEDO, O; ZDRAHAL, Z; COSTANZO, G; PINO, S; SALADINO, R; DI MAURO, E, 2016:Emergence of the First Catalytic Oligonucleotides in a Formamide-Based Origin Scenario. CHEMISTRY-A EUROPEAN JOURNAL 22 (11), p. 3572 - 3586.
• STOKLASOVA, M; KUCHAROVA, H; BURDOVA, A; DREVOJANKOVA, B; SABLATUROVA, T; STRNKOVA, A; TRUHLIKOVA, L; YVONA, B; PANOVSKA, A; PLEVOVA, K; OLTOVA, A; RYTIKOVA, N; PETRICOVA, N; ADAMOVA, D; HEINZOVA, V; ZYGULOVA, I; LASKA, J; ZUCHNICKA, J; BODZASOVA, C; DURAS, J; URBANKOVA, M; JEHLIKOVA, Z; SLEZAK, P; MACUROVA, J; JANEK, D; BOGOCZOVA, E; WROBEL, M; KLODOVA, D; ZETKOVA, Z; BREJCHA, M, 2016:RARE RECURRENT CHROMOSOMAL ABNORMALITIES IN CLL DETECTED BY CONVENTIONAL CYTOGENETICS AFTER STIMULATION WITH CPG-ODN & IL-2 AND FISH. HAEMATOLOGICA 101 , p. 717 - 717.
• SUTTON, LA; YOUNG, E; BALIAKAS, P; HADZIDIMITRIOU, A; MOYSIADIS, T; PLEVOVA, K; ROSSI, D; KMINKOVA, J; STALIKA, E; PEDERSEN, LB; MALCIKOVA, J; AGATHANGELIDIS, A; DAVIS, Z; MANSOURI, L; SCARFO, L; BOUDJOGHRA, M; NAVARRO, A; MUGGEN, AF; YAN, XJ; NGUYEN-KHAC, F; LARRAYOZ, M; PANAGIOTIDIS, P; CHIORAZZI, N; NIEMANN, CU; BELESSI, C; CAMPO, E; STREFFORD, JC; LANGERAK, AW; OSCIER, D; GAIDANO, G; POSPISILOVA, S; DAVI, F; GHIA, P; STAMATOPOULOS, K; ROSENQUIST, R, 2016:Different spectra of recurrent gene mutations in subsets of chronic lymphocytic leukemia harboring stereotyped B-cell receptors. HAEMATOLOGICA 101 (8), p. 959 - 967.
• TURCHANINOVA, MA; DAVYDOV, A; BRITANOVA, OV; SHUGAY, M; BIKOS, V; EGOROV, ES; KIRGIZOVA, VI; MERZLYAK, EM; STAROVEROV, DB; BOLOTIN, DA; MAMEDOV, IZ; IZRAELSON, M; LOGACHEVA, MD; KLADOVA, O; PLEVOVA, K; POSPISILOVA, S; CHUDAKOV, DM, 2016:High-quality full-length immunoglobulin profiling with unique molecular barcoding. NATURE PROTOCOLS 11 (9), p. 1599 - 1616.
• VYCHYTILOVA-FALTEJSKOVA, P; RADOVA, L; SACHLOVA, M; KOSAROVA, Z; SLABA, K; FABIAN, P; GROLICH, T; PROCHAZKA, V; KALA, Z; SVOBODA, M; KISS, I; VYZULA, R; SLABY, O, 2016:Serum-based microRNA signatures in early diagnosis and prognosis prediction of colon cancer. CARCINOGENESIS 37 (10), p. 941 - 950.
• ZAPRAZNA, K; MALCIKOVA, J; TOM, N; RADOVA, L; TRBUSEK, M; ATCHISON, M; POSPISILOVA, S, 2016:ACTIVATION INDUCED DEAMINASE SPLICE VARIANTS EXPRESSED IN B CELL LEUKEMIA AND LYMPHOMA DO NOT RETAIN THEIR CATALYTIC ACTIVITY. HAEMATOLOGICA 101 , p. 429 - 430.

2015

• AGATHANGELIDIS, A; BYSTRY, V; HADZIDIMITRIOU, A; SUTTON, LA; MINGA, E; KIENLE, D; DAVIS, Z; YAN, XJ; SHANAFELT, T; BOUDJOGRA, M; PLEVOVA, K; GOUNARI, M; XOCHELLI, A; NAVARRO, A; CHATZOULI, M; PEDERSEN, LB; BALIAKAS, P; SCARFO, L; ROSSI, D; VERONESE, S; FACCO, M; BIKOS, V; KARAN-DJURASEVIC, T; PAVLOVIC, S; MANSOURI, L; POIRON, C; CHU, CC; STALIKA, E; GIUDICELLI, V; PANAGIOTIDIS, P; SUDARIKOV, A; ANAGNOSTOPOULOS, A; TRENTIN, L; CATHERWOOD, M; MONTILLO, M; GAIDANO, G; CAMPO, E; GEISLER, CH; LANGERAK, AW; POSPISILOVA, S; LEFRANC, MP; CHIORAZZI, N; OSCIER, D; JELINEK, DF; STILGENBAUER, S; BELESSI, C; DAVI, F; ROSENQUIST, R; GHIA, P; DARZENTAS, N; STAMATOPOULOS, K, 2015:HIGHER-ORDER IMMUNOGLOBULIN SEQUENCE RELATIONS FOR MAJOR SUBSETS OF CHRONIC LYMPHOCYTIC LEUKEMIA: UNIQUENESS VERSUS EQUIVALENCE. HAEMATOLOGICA 100 , p. 47 - 48.
• BALIAKAS, P; AGATHANGELIDIS, A; HADZIDIMITRIOU, A; SUTTON, LA; MINGA, E; TSANOUSA, A; SCARFO, L; DAVIS, Z; YAN, XJ; SHANAFELT, T; PLEVOVA, K; SANDBERG, Y; VOJDEMAN, FJ; BOUDJOGRA, M; TZENOU, T; CHATZOULI, M; CHU, CC; VERONESE, S; GARDINER, A; MANSOURI, L; SMEDBY, KE; PEDERSEN, LB; MORENO, D; VAN LOM, K; GIUDICELLI, V; FRANCOVA, HS; NGUYEN-KHAC, F; PANAGIOTIDIS, P; JULIUSSON, G; ANGELIS, L; ANAGNOSTOPOULOS, A; LEFRANC, MP; FACCO, M; TRENTIN, L; CATHERWOOD, M; MONTILLO, M; GEISLER, CH; LANGERAK, AW; POSPISILOVA, S; CHIORAZZI, N; OSCIER, D; JELINEK, DF; DARZENTAS, N; BELESSI, C; DAVI, F; GHIA, P; ROSENQUIST, R; STAMATOPOULOS, K, 2015:Not all IGHV3-21 chronic lymphocytic leukemias are equal: prognostic considerations. BLOOD 125 (5), p. 856 - 859.
• BALIAKAS, P; HADZIDIMITRIOU, A; AGATHANGELIDIS, A; ROSSI, D; SUTTON, LA; KMINKOVA, J; SCARFO, L; POSPISILOVA, S; GAIDANO, G; STAMATOPOULOS, K; GHIA, P; ROSENQUIST, R, 2015:Prognostic relevance of MYD88 mutations in CLL: the jury is still out. BLOOD 126 (8), p. 1043 - 1044.
• BALIAKAS, P; HADZIDIMITRIOU, A; MATTSSON, M; XOCHELLI, A; SUTTON, LA; MINGA, E; SCARFO, L; ROSSI, D; DAVIS, Z; AGATHANGELIDIS, A; VILLAMOR, N; PARKER, H; KOTASKOVA, J; STALIKA, E; PLEVOVA, K; MANSOURI, L; CORTESE, D; LOPEZ, AN; DELGADO, J; LARRAYOZ, M; ANAGNOSTOPOULOS, A; BELESSI, C; SMEDBY, KE; JULIUSSON, G; STREFFORD, JC; POSPISILOVA, S; OSCIER, D; GAIDANO, G; CAMPO, E; GHIA, P; ROSENQUIST, R; STAMATOPOULOS, K, 2015:REFINING PROGNOSIS OF CHRONIC LYMPHOCYTIC LEUKEMIA WITH SOMATICALLY HYPERMUTATED B-CELL RECEPTORS: A NOVEL PROGNOSTIC INDEX ON BEHALF OF THE EUROPEAN RESEARCH INITIATIVE ON CLL (ERIC). HAEMATOLOGICA 100 , p. 52 - 52.
• BALIAKAS, P; HADZIDIMITRIOU, A; SUTTON, LA; ROSSI, D; MINGA, E; VILLAMOR, N; LARRAYOZ, M; KMINKOVA, J; AGATHANGELIDIS, A; DAVIS, Z; TAUSCH, E; STALIKA, E; KANTOROVA, B; MANSOURI, L; SCARFO, L; CORTESE, D; NAVRKALOVA, V; ROSE-ZERILLI, MJJ; SMEDBY, KE; JULIUSSON, G; ANAGNOSTOPOULOS, A; MAKRIS, AM; NAVARRO, A; DELGADO, J; OSCIER, D; BELESSI, C; STILGENBAUER, S; GHIA, P; POSPISILOVA, S; GAIDANO, G; CAMPO, E; STREFFORD, JC; STAMATOPOULOS, K; ROSENQUIST, R, 2015:Recurrent mutations refine prognosis in chronic lymphocytic leukemia. LEUKEMIA 29 (2), p. 329 - 336.
• BALIAKAS, P; PUIGGROS, A; XOCHELLI, A; SUTTON, LA; NGUYEN-KHAC, F; GARDINER, A; PLEVOVA, K; ORTEGA, M; COLLADO, R; GONZALEZ, T; GRANADA, I; LUNO, E; KOTASKOVA, J; DAVIS, Z; ANAGNOSTOPOULOS, A; STREFFORD, J; POSPISILOVA, S; DAVI, F; ATHANASIADOU, A; ROSENQUIST, R; OSCIER, D; SOLA, BE; STAMATOPOULOS, K, 2015:ADDITIONAL TRISOMIES AMONGST PATIENTS WITH CHRONIC LYMPHOCYTIC LEUKEMIA CARRYING TRISOMY 12: THE PARTNER CHROMOSOME MAKES A DIFFERENCE. HAEMATOLOGICA 100 , p. 224 - 224.
• BESSE, L; SEDLARIKOVA, L; KRYUKOV, F; NEKVINDOVA, J; RADOVA, L; SLABY, O; KUGLIK, P; ALMASI, M; PENKA, M; KREJCI, M; ADAM, Z; POUR, L; SEVCIKOVA, S; HAJEK, R, 2015:Circulating Serum MicroRNA-130a as a Novel Putative Marker of Extramedullary Myeloma. PLOS ONE 10 (9)
• BRAZDILOVA, K; PLEVOVA, K; FRANCOVA, HS; KOCKOVA, H; CHMELIKOVA, M; BORSKY, M; BURCKOVA, K; KANTOROVA, B; TICHY, B; SKABRAHOVA, H; BRYCHTOVA, Y; MAYER, J; DOUBEK, M; POSPISILOVA, S, 2015:Single Cell Analysis of IG Genes in CLL: Cases with Multiple IGH Rearrangements Are Constituted of Several Independent Clones Even When Indistinguishable By Flow Cytometry. BLOOD 126 (23)
• BRUGGERMANN, M; KNECHT, H; BARTRAM, J; BYSTRY, V; CAZZANIGA, G; FAZIO, G; FERRERO, S; FRONKOVA, E; GARCIA-SANZ, R; GRIONI, A; HANCOCK, J; JIMINEZ, C; KOTROVA, M; LADETTO, M; MOPPETT, J; SONGIA, S; TRKA, J; LANGERAK, AW; DARZENTAS, N; HERRMANN, D; POTT, C, 2015:INTERNATIONAL MULTI-LABORATORY NEXT-GENERATION SEQUENCING FOR MRD ANALYSIS IN ALL. A PILOT STUDY BY THE EUROCLONALITY-NGS CONSORTIUM. HAEMATOLOGICA 100 , p. 31 - 31.
• BYSTRY, V; AGATHANGELIDIS, A; BIKOS, V; SUTTON, LA; BALIAKAS, P; HADZIDIMITRIOU, A; STAMATOPOULOS, K; DARZENTAS, N, 2015:ARResT/AssignSubsets: a novel application for robust subclassification of chronic lymphocytic leukemia based on B cell receptor IG stereotypy. BIOINFORMATICS 31 (23), p. 3844 - 3846.
• DOUBEK, M; SMIDA, M, 2015:Treatment of chronic lymphocytic leukemia with monoclonal antibodies, where are we heading?. EXPERT REVIEW OF HEMATOLOGY 8 (6), p. 743 - 764.
• CHATZILARI, E; BALIAKAS, P; XOCHELLI, A; MARONIDIS, A; VARDI, A; MATTSSON, M; LARSSON, K; DOUKA, V; ISKAS, M; KARAVALAKIS, G; PAPALEXANDRI, A; NIEMANN, C; MONTILLO, M; ANAGNOSTOPOULOS, A; OSCIER, D; POSPISILOVA, S; DAVI, F; STAVROYIANNI, N; GHIA, P; HADZIDIMITRIOU, A; ROSENQUIST, R; NIKOLOPOULOS, S; STAMATOPOULOS, K; KOMPATSIARIS, Y, 2015:Personalized Modeling of Disease Evolution in CLL: Does Statistical Significance Translate into Predictive Accuracy?. BLOOD 126 (23)
• CHU, VH; PARK, LP; ATHAN, E; DELAHAYE, F; FREIBERGER, T; LAMAS, C; MIRO, JM; MUDRICK, DW; STRAHILEVITZ, J; TRIBOUILLOY, C; DURANTE-MANGONI, E; PERICAS, JM; FERNANDEZ-HIDALGO, N; NACINOVICH, F; RIZK, H; KRAJINOVIC, V; GIANNITSIOTI, E; HURLEY, JP; HANNAN, MM; WANG, A, 2015:Association Between Surgical Indications, Operative Risk, and Clinical Outcome in Infective Endocarditis A Prospective Study From the International Collaboration on Endocarditis. CIRCULATION 131 (2), p. 131 - U46.
• CHU, VH; PARK, LP; ATHAN, E; DELAHAYE, F; FREIBERGER, T; LAMAS, C; MIRO, JM; MUDRICK, DW; STRAHILEVITZ, J; TRIBOUILLOY, C; DURANTE-MANGONI, E; PERICAS, JM; FERNANDEZ-HIDALGO, N; NACINOVICH, F; RIZK, H; KRAJINOVIC, V; GIANNITSIOTI, E; HURLEY, JP; HANNAN, MM; WANG, A, 2015:Response to Letter Regarding Article, "Association Between Surgical Indications, Operative Risk, and Clinical Outcome in Infective Endocarditis: A Prospective Study From the International Collaboration on Endocarditis". CIRCULATION 132 (14), p. E184 - E185.
• JANOUSKOVA, E; NECASOVA, I; PAVLOUSKOVA, J; ZIMMERMANN, M; HLUCHY, M; MARINI, V; NOVAKOVA, M; HOFR, C, 2015:Human Rap1 modulates TRF2 attraction to telomeric DNA. NUCLEIC ACIDS RESEARCH 43 (5), p. 2691 - 2700.
• JASKOVA, Z; MALCIKOVA, J; SMARDOVA, J; BRYCHTOVA, Y; MAYER, J; TRBUSEK, M, 2015:ELIMINATION OF MUTATED P53 PROTEIN AND INDUCTION OF P53 DOWNSTREAM GENES IN CHRONIC LYMPHOCYTIC LEUKEMIA CELLS USING PRIMA-1MET. HAEMATOLOGICA 100 , p. 414 - 414.
• KANTOROVA, B; MALCIKOVA, J; BRAZDILOVA, K; BORSKY, M; PLEVOVA, K; SMARDOVA, J; TRBUSEK, M; BRYCHTOVA, Y; DOUBEK, M; MAYER, J; POSPISILOVA, S, 2015:Single Cell Analysis Proves the Coexistence of NOTCH1 and TP53 Mutations within the Same Cancer Cells in Patients with Chronic Lymphocytic Leukemia. BLOOD 126 (23)
• KOTASKOVA, J; MALCIKOVA, J; DIVISKOVA, E; PAVLOVA, S; MEJSTRIKOVA, S; VRANOVA, V; PLEVOVA, K; PLESINGEROVA, H; DOUBEK, M; MAYER, J; POSPISILOVA, S, 2015:13Q DELETION IS PREDOMINANT CYTOGENETIC ABERRATION NEWLY AQUIRED DURING CHRONIC LYMPHOCYTIC LEUKEMIA COURSE IRRESPECTIVE OF DISEASE ACTIVITY AND TREATMENT STATUS. HAEMATOLOGICA 100 , p. 417 - 418.
• KOTROVA, M; KNECHT, H; BARTRAM, J; BYSTRY, V; CAZZANIGA, G; FAZIO, G; FERRERO, S; GENUARDI, E; GARCIA-SANZ, R; GRIONI, A; HANCOCK, J; JIMINEZ, C; LADETTO, M; MOPPETT, J; SONGIA, S; HERRMANN, D; POTT, C; LANGERAK, AW; DARZENTAS, N; TRKA, J; FRONKOVA, E; BRUGGEMANN, M, 2015:Library Preparation Is the Major Factor Affecting Differences in Results of Immunoglobulin Gene Rearrangements Detection on Two Major Next-Generation Sequencing Platforms. BLOOD 126 (23)
• KRYUKOV, F; NEMEC, P; RADOVA, L; KRYUKOVA, E; KUFOVA, Z; SEDLARIKOVA, L; SEVCIKOVA, S; HAJEK, R, 2015:CENTROSOME ASSOCIATED GENES PATTERN FOR RISK SUBSTRATIFICATION IN MULTIPLE MYELOMA. HAEMATOLOGICA 100 , p. 247 - 247.
• LJUNGSTROM, V; CORTESE, D; YOUNG, E; PANDZIC, T; MANSOURI, L; PLEVOVA, K; SUTTON, LA; STAVROYIANNI, N; AGATHANGELIDIS, A; ROSSI, D; HOGLUND, M; OSCIER, D; GAIDANO, G; DAVI, F; POTT, C; TRENTIN, L; POSPISILOVA, S; GHIA, P; STAMATOPOULOS, K; SJOBLOM, T; ROSENQUIST, R, 2015:DISSECTING RESISTANCE MECHANISMS IN CHRONIC LYMPHOCYTIC LEUKEMIA USING WHOLE-EXOME SEQUENCING: IMPACT OF RECURRENT RPS15 MUTATIONS ON P53 DYSREGULATION. HAEMATOLOGICA 100 , p. 10 - 11.
• MALCIKOVA, J; STANO-KOZUBIK, K; TICHY, B; KANTOROVA, B; PAVLOVA, S; TOM, N; RADOVA, L; SMARDOVA, J; PARDY, F; DOUBEK, M; BRYCHTOVA, Y; MRAZ, M; PLEVOVA, K; DIVISKOVA, E; OLTOVA, A; MAYER, J; POSPISILOVA, S; TRBUSEK, M, 2015:Detailed analysis of therapy-driven clonal evolution of TP53 mutations in chronic lymphocytic leukemia. LEUKEMIA 29 (4), p. 877 - 885.
• MANSOURI, L; SUTTON, LA; LJUNGSTROM, V; BONDZA, S; ARNGARDEN, L; BHOI, S; LARSSON, J; CORTESE, D; KALUSHKOVA, A; PLEVOVA, K; YOUNG, E; GUNNARSSON, R; FALK-SORQVIST, E; LONN, P; MUGGEN, AF; YAN, XJ; SANDER, B; ENBLAD, G; SMEDBY, KE; JULIUSSON, G; BELESSI, C; RUNG, J; CHIORAZZI, N; STREFFORD, JC; LANGERAK, AW; POSPISILOVA, S; DAVI, F; HELLSTROM, M; JERNBERG-WIKLUND, H; GHIA, P; SODERBERG, O; STAMATOPOULOS, K; NILSSON, M; ROSENQUIST, R, 2015:Functional loss of I kappa B epsilon leads to NF-kappa B deregulation in aggressive chronic lymphocytic leukemia. JOURNAL OF EXPERIMENTAL MEDICINE 212 (6), p. 833 - 843.
• MLCOCHOVA, J; FALTEJSKOVA-VYCHYTILOVA, P; FERRACIN, M; ZAGATTI, B; RADOVA, L; SVOBODA, M; NEMECEK, R; JOHN, S; KISS, I; VYZULA, R; NEGRINI, M; SLABY, O, 2015:MicroRNA expression profiling identifies miR-31-5p/3p as associated with time to progression in wild-type RAS metastatic colorectal cancer treated with cetuximab. ONCOTARGET 6 (36), p. 38695 - 38704.
• MUSILOVA, K; MRAZ, M, 2015:MicroRNAs in B-cell lymphomas: how a complex biology gets more complex. LEUKEMIA 29 (5), p. 1004 - 1017.
• NAVRKALOVA, V; KAFKOVA, LR; DIVOKY, V; POSPISILOVA, S, 2015:Oxidative stress as a therapeutic perspective for ATM-deficient chronic lymphocytic leukemia patients. HAEMATOLOGICA 100 (8), p. 994 - 996.
• NAVRKALOVA, V; YOUNG, E; BALIAKAS, P; RADOVA, L; PLEVOVA, K; SUTTON, LA; MANSOURI, L; LJUNGSTROM, V; NTOUFA, S; DAVIS, Z; JULIUSSON, G; SMEDBY, KE; BELESSI, C; PANAGIOTIDIS, P; DAVI, F; LANGERAK, AW; GHIA, P; STREFFORD, JC; OSCIER, DG; STAMATOPOULOS, K; POSPISILOVA, S; ROSENQUIST, R; TRBUSEK, M, 2015:ATM Mutations in Major Stereotyped CLL Subsets: Enrichment in Subset #2 is Associated with Unfavourable Outcome. BLOOD 126 (23)
• NEMEC, A; KRIZOVA, L; MAIXNEROVA, M; SEDO, O; BRISSE, S; HIGGINS, PG, 2015:Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65 , p. 934 - 942.
• NOERENBERG, D; YOUNG, E; LJUNGSTROM, V; MANSOURI, L; PLEVOVA, K; BALIAKAS, P; BLAKEMORE, S; ROSSI, D; CLIFFORD, R; NAVRKALOVA, V; SUTTON, LA; SMEDBY, KE; JULIUSSON, G; BELESSI, C; PANAGIOTIDIS, P; CHIORAZZI, N; DAVI, F; LANGERAK, AW; SCHUH, A; GAIDANO, G; STREFFORD, JC; GHIA, P; STAMATOPOULOS, K; POSPISILOVA, S; ZENZ, T; ROSENQUIST, R; DAMM, F, 2015:EGR2 Mutations in Chronic Lymphocytic Leukemia: A New Bad Player. BLOOD 126 (23)
• PAVLASOVA, G; SEDA, V; BORSKY, M; CERNA, K; OSICKOVA, J; MAYER, J; POSPISILOVA, S; DAVIDS, MS; BROWN, JR; MRAZ, M, 2015:Microenvironmental Interactions up-Regulate CD20 Expression in CLL B Cells through the CXCR4/SDF-1 Axis: Implications for CD20-Targeting Antibodies and the Use of BCR-Inhibitors in Combination. BLOOD 126 (23)
• PLESINGEROVA, H; JANOVSKA, P; POPPOVA, L; SHAIK, A; JURCICKOVA, Z; LIBRA, A; PLEVOVA, K; MACHAC, J; SLAPAK, I; PAVLOVA, S; OVESNA, P; KOTASKOVA, J; DOUBEK, M; POSPISILOVA, S; BRYJA, V, 2015:UPREGULATED COBLL1 IN CHRONIC LYMPHOCYTIC LEUKAEMIA (CLL) PATIENTS WITH UNMUTATED IGHV IDENTIFIES A COHORT WITH INFERIOR PROGNOSIS. HAEMATOLOGICA 100 , p. 50 - 50.
• PUDJA, A; MILOSEVIC, JD; KLAMPFL, T; HARUTYUNYAN, A; BERG, T; BAGIENSKI, K; CHEN, D; GISSLINGER, B; RUMI, E; MALCOVATI, L; PIETRA, D; ELENA, C; DELLA PORTA, MG; PIERI, L; GUGLIELMELLI, P; DOUBEK, M; DVORAKOVA, D; SUVAJDZIC, N; TOMIN, D; TOSIC, N; RACIL, Z; STEURER, M; PAVLOVIC, S; VANNUCCHI, AM; CAZZOLA, M; GISSLINGER, H; KRALOVICS, R, 2015:HIGH RESOLUTION CYTOGENETIC MAPPING AND WHOLE EXOME SEQUENCING REVEAL A COMPLEX PATTERN OF CHROMOSOME 6P ABERRATIONS IN PATIENTS WITH MYELOID MALIGNANCIES. HAEMATOLOGICA 100 , p. 97 - 97.
• REBLOVA, M; JAKLITSCH, WM; REBLOVA, K; STEPANEK, V, 2015:Phylogenetic Reconstruction of the Calosphaeriales and Togniniales Using Five Genes and Predicted RNA Secondary Structures of ITS, and Flabellascus tenuirostris gen. et sp nov.. PLOS ONE 10 (12)
• SEDA, V; MRAZ, M, 2015:B-cell receptor signalling and its crosstalk with other pathways in normal and malignant cells. EUROPEAN JOURNAL OF HAEMATOLOGY 94 (3), p. 193 - 205.
• SELLNER, L; DREGER, P; SCHLITT, M; KNECHT, H; HERRMANN, D; REIGL, T; KREJCI, A; BYSTRY, V; DARZENTAS, N; RIEGER, M; DIETRICH, S; ZENZ, T; HO, AD; KNEBA, M; BRUEGGEMANN, M, 2015:T-Prolymphocytic Leukemia Is Sensitive to Polyclonal T Cell-Derived Graft-Versus-Leukemia Effects: Evidence from Minimal Residual Disease Kinetics and TCR Repertoire Diversity Analyses. BLOOD 126 (23)
• SUTTON, LA; LJUNGSTROM, V; MANSOURI, L; YOUNG, E; CORTESE, D; NAVRKALOVA, V; MALCIKOVA, J; MUGGEN, AF; TRBUSEK, M; PANAGIOTIDIS, P; DAVI, F; BELESSI, C; LANGERAK, AW; GHIA, P; POSPISILOVA, S; STAMATOPOULOS, K; ROSENQUIST, R, 2015:Targeted next-generation sequencing in chronic lymphocytic leukemia: a high-throughput yet tailored approach will facilitate implementation in a clinical setting. HAEMATOLOGICA 100 (3), p. 370 - 376.
• TE RAA, GD; DERKS, IAM; NAVRKALOVA, V; SKOWRONSKA, A; MOERLAND, PD; VAN LAAR, J; OLDREIVE, C; MONSUUR, H; TRBUSEK, M; MALCIKOVA, J; LODEN, M; GEISLER, CH; HULLEIN, J; JETHWA, A; ZENZ, T; POSPISILOVA, S; STANKOVIC, T; VAN OERS, MHJ; KATER, AP; ELDERING, E, 2015:The impact of SF3B1 mutations in CLL on the DNA-damage response. LEUKEMIA 29 (5), p. 1133 - 1142.
• TOM, N; MALCIKOVA, J; RADOVA, L; KANTOROVA, B; PARDY, F; PAVLOVA, S; PAL, K; MRAZ, M; TICHY, B; DOUBEK, M; BRYCHTOVA, Y; PLEVOVA, K; MAYER, J; TRBUSEK, M; POSPISILOVA, S, 2015:MUTATIONS IN THE TP53 GENE SHOW FEATURES OF SOMATIC HYPERMUTATION PROCESS WITH PROMINENT DIFFERENCE BETWEEN IGHV MUTATED AND UNMUTATED CHRONIC LYMPHOCYTIC LEUKEMIA. HAEMATOLOGICA 100 , p. 224 - 225.
• VARDI, A; KARIPIDOU, M; GEMENETZI, A; VLACHONIKOLA, E; BIKOS, V; STALIKA, E; KORAVOU, E; MARAMIS, C; SIORENTA, A; ANAGNOSTOPOULOS, A; POSPISILOVA, S; MAGLAVERAS, N; CHOUVARDA, I; STAMATOPOULOS, K; HADZIDIMITRIOU, A, 2015:Next Generation Sequence Immunoprofiling of the T-Cell Repertoire in Chronic Lymphocytic Leukemia Supports Selection By Shared Antigenic Elements. BLOOD 126 (23)
• VARDI, A; STALIKA, E; GKOUFAS, A; KARIPIDOU, M; MARAMIS, C; BIKOS, V; POSPISILOVA, S; ANAGNOSTOPOULOS, A; MAGLAVERAS, N; CHOUVARDA, I; STAMATOPOULOS, K; HADZIDIMITRIOU, A, 2015:HIGH-THROUGHPUT T-CELL RECEPTOR GENE REPERTOIRE PROFILING IN CHRONIC LYMPHOCYTIC LEUKEMIA SUBSET #4: FURTHER EVIDENCE OF ANTIGENIC STIMULATION. HAEMATOLOGICA 100 , p. 11 - 12.
• XOCHELLI, A; BIKOS, V; POLYCHRONIDOU, E; AGATHANGELIDIS, A; CHARLOTTE, F; MOSCHONAS, P; DAVIS, Z; COLOMBO, M; ROUMELIOTI, M; SUTTON, LA; GROENEN, P; BOUDJOGHRA, M; ALGARA, P; TRAVERSE-GLEHEN, A; FERRER, A; STALIKA, E; KARYPIDOU, M; KANELLIS, G; KALPADAKIS, C; MOLLEJO, M; PANGALIS, G; VLAMOS, P; AMINI, RM; POSPISILOVA, S; GONZALEZ, D; PONZONI, M; ANAGNOSTOPOULOS, A; GIUDICELLI, V; LEFRANC, MP; ESPINET, B; PANAGIOTIDIS, P; PIRIS, MA; DU, M; ROSENQUIST, R; PAPADAKI, T; BELESSI, C; FERRARINI, M; OSCIER, D; TZOVARAS, D; GHIA, P; DAVI, F; HADZIDIMITRIOU, A; STAMATOPOULOS, K, 2015:Unique Versus Common: Disease-Biased Immunoglobulin Gene Repertoires Along with Public Antigen Receptor Stereotypes in Marginal Zone B-Cell Lymphoproliferations. BLOOD 126 (23)
• ZEMANOVA, J; PARUCH, K; KREJCI, L; SOUCEK, K; HYLSE, O; BOUDNY, M; BORSKY, M; OSICKOVA, J; KHIRSARIYA, P; SEBEJOVA, L; NAVRKALOVA, V; MALCIKOVA, J; DIVISKOVA, E; BRYCHTOVA, Y; MAYER, J; TRBUSEK, M, 2015:CHRONIC LYMPHOCYTIC LEUKEMIA CELLS ARE HIGHLY SUSCEPTIBLE TO DIRECT INHIBITION OF CHECKPOINT KINASE 1. HAEMATOLOGICA 100 , p. 417 - 417.

2014

• BALAZOVA, T; MAKOVCOVA, J; SEDO, O; SLANY, M; FALDYNA, M; ZDRAHAL, Z, 2014:The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling. FEMS MICROBIOLOGY LETTERS 353 (1), p. 77 - 84.
• BALIAKAS, P; HADZIDIMITRIOU, A; SUTTON, LA; MINGA, E; AGATHANGELIDIS, A; TSANOUSA, A; SCARFO, L; DAVIS, Z; YAN, J; SHANAFELT, TD; PLEVOVA, K; SANDBERG, Y; VOJDEMAN, FJ; BOUDJOGHRA, M; TZENOU, T; CHATZOULI, M; CHU, CC; VERONESE, S; GARDINER, AC; MANSOURI, L; SMEDBY, KE; PEDERSEN, L; MORENO, D; VAN LOM, K; GIUDICELLI, V; FRANCOVA, H; NGUYEN-KHAC, F; PANAGIOTIDIS, P; JULIUSSON, G; ANGELIS, L; ANAGNOSTOPOULOS, A; LEFRANC, MP; FACCO, M; TRENTIN, L; CATHERWOOD, M; MONTILLO, M; GEISLER, CH; LANGERAK, AW; POSPISILOVA, S; CHIORAZZI, N; OSCIER, D; JELINEK, DF; DARZENTAS, N; BELESSI, C; DAVI, F; ROSENQUIST, R; GHIA, P; STAMATOPOULOS, K, 2014:Clinical Impact of Stereotyped Antigen Receptors in Chronic Lymphocytic Leukemia. BLOOD 124 (21)
• BALIAKAS, P; HADZIDIMITRIOU, A; SUTTON, LA; MINGA, E; AGATHANGELIDIS, A; TSANOUSA, A; SCARFO, L; DAVIS, Z; YAN, XJ; SHANAFELT, T; PLEVOVA, K; SANDBERG, Y; VOJDEMAN, FJ; BOUDJOGRA, M; TZENOU, T; CHATZOULI, M; CHU, CC; VERONESE, S; GARDINER, A; MANSOURI, L;, 2014:NOT ALL IGHV3-21 CLL ARE EQUAL: SUBSET #2 DISPLAYS A DISTINCTIVE CLINICOBIOLOGICAL PROFILE WITH REMARKABLE SIMILARITIES TO SUBSET #169, ITS CLOSE IMMUNOGENETIC RELATIVE. HAEMATOLOGICA 99 , p. 48 - 49.
• DE GROOT, REA; GANJI, RS; BERNATIK, O; LLOYD-LEWIS, B; SEIPEL, K; SEDOVA, K; ZDRAHAL, Z; DHOPLE, VM; DALE, TC; KORSWAGEN, HC; BRYJA, V, 2014:Huwe1-Mediated Ubiquitylation of Dishevelled Defines a Negative Feedback Loop in the Wnt Signaling Pathway. SCIENCE SIGNALING 7 (317)
• DOUBEK, M; SROVNAL, J; PLEVOVA, K; TRIZULJAK, J; BRYCHTOVA, Y; SEMERAD, L; LETALOVA, E; BENEDIKOVA, A; MAYER, J; HAJDUCH, M; POSPISILOVA, S, 2014:ANALYSIS OF MERKEL CELL POLYOMAVIRUS PROGNOSTIC SIGNIFICANCE IN CHRONIC LYMPHOCYTIC LEUKEMIA. HAEMATOLOGICA 99 , p. 325 - 325.
• HOFFMANN, V; LINDOERFER, D; THALER, J; LABAR, B; MELANTHIOU, F; MAYER, J; EVERAUS, H; PORKKA, K; GUILLHOT, J; SCHUBERT-FRITSCHLE, G; CASTAGNETTI, F; LEJNIECE, S; GRISKEVICIUS, L; THIELEN, N; HELLMANN, A; TURKINA, A; ZARITSKEY, A; BOGDANOVIC, AD; INDRAK, K, 2014:THE EUTOS POPULATION BASED REGISTRY - INCIDENCES OF CML ACROSS EUROPE. HAEMATOLOGICA 99 , p. 72 - 72.
• LENGEROVA, M; RACIL, Z; HRNCIROVA, K; KOCMANOVA, I; VOLFOVA, P; RICNA, D; BEJDAK, P; MOULIS, M; PAVLOVSKY, Z; WEINBERGEROVA, B; TOSKOVA, M; MAYER, J, 2014:Rapid Detection and Identification of Mucormycetes in Bronchoalveolar Lavage Samples from Immunocompromised Patients with Pulmonary Infiltrates by Use of High-Resolution Melt Analysis. JOURNAL OF CLINICAL MICROBIOLOGY 52 (8), p. 2824 - 2828.
• LINDOERFER, D; HOFFMANN, VS; ROSTI, G; CASTAGNETTI, F; SAUSSELE, S; GUILHOT, J; SIMONSSON, B; STEEGMANN, JL; MAYER, J; INDRAK, K; TURKINA, AG; ZARITSKEY, A; LABAR, B; ZUPAN, IP; THIELEN, N; CLARK, RE; THALER, J; MELANTHIOU, F; EVERAUS, H; PORKKA, K; BOGDA, 2014:THE EUTOS POPULATION-BASED REGISTRY: EVALUATION OF BASELINE CHARACTERISTICS AND FIRST TREATMENT CHOICES OF 2983 NEWLY DIAGNOSED CHRONIC MYELOID LEUKEMIA (CML) PATIENTS FROM 20 EUROPEAN COUNTRIES. HAEMATOLOGICA 99 , p. 238 - 239.
• NAVRKALOVA, V; MARECKOVA, A; JANIKOVA, A; DOUBEK, M; MAYER, J; POSPISILOVA, S; TRBUSEK, M, 2014:INCORPORATION OF NEXT-GENERATION SEQUENCING INTO ATM GENE ANALYSIS IN PATIENTS WITH CLL AND MCL. HAEMATOLOGICA 99 , p. 312 - 312.
• POLANSKA, E; POSPISILOVA, S; STROS, M, 2014:Binding of Histone H1 to DNA Is Differentially Modulated by Redox State of HMGB1. PLOS ONE 9 (2)
• ROBESOVA, B; BAJEROVA, M; LISKOVA, K; SKRICKOVA, J; TOMISKOVA, M; POSPISILOVA, S; MAYER, J; DVORAKOVA, D, 2014:TaqMan based real time PCR assay targeting EML4-ALK fusion transcripts in NSCLC. LUNG CANCER 85 (1), p. 25 - 30.
• SHUGAY, M; BRITANOVA, OV; MERZLYAK, EM; TURCHANINOVA, MA; MAMEDOV, IZ; TUGANBAEV, TR; BOLOTIN, DA; STAROVEROV, DB; PUTINTSEVA, EV; PLEVOVA, K; LINNEMANN, C; SHAGIN, D; POSPISILOVA, S; LUKYANOV, S; SCHUMACHER, TN; CHUDAKOV, DM, 2014:Towards error-free profiling of immune repertoires. NATURE METHODS 11 (6), p. 653 - +.
• SUTTON, LA; LJUNGSTROM, V; MANSOURI, L; YOUNG, E; CORTESE, D; NAVRKALOVA, V; MALCIKOVA, J; MUGGEN, AF; TRBUSEK, M; DAVI, F; BELESSI, C; LANGERAK, AW; GHIA, P; POSPISILOVA, S; STAMATOPOULOS, K; ROSENQUIST, R, 2014:TARGETED NEXT-GENERATION SEQUENCING FOR MUTATIONAL SCREENING IN CHRONIC LYMPHOCYTIC LEUKEMIA: A HIGH-THROUGHPUT YET TAILORED APPROACH WILL FACILITATE IMPLEMENTATION WITHIN A CLINICAL SETTING. HAEMATOLOGICA 99 , p. 313 - 314.
• SUTTON, LA; MOSCHONAS, P; VARDI, A; BIKOS, V; YAN, XJ; CHATZOULI, M; ANAGNOSTOPOULOS, A; BELESSI, C; CHIORAZZI, N; ROSENQUIST, R; TZOVARAS, D; STAMATOPOULOS, K; HADZIDIMITRIOU, A, 2014:MATCHED PATTERN DISCOVERY ACROSS PAIRED IMMUNOGLOBULIN HEAVY AND LIGHT CHAINS IN CLL REVEALS UNIQUE SUBSET-DEFINING AMINO ACID ASSOCIATIONS. HAEMATOLOGICA 99 , p. 50 - 50.
• VARDI, A; AGATHANGELIDIS, A; SUTTON, LA; CHATZOULI, M; SCARFO, L; MANSOURI, L; DOUKA, V; ANAGNOSTOPOULOS, A; DARZENTAS, N; ROSENQUIST, R; GHIA, P; BELESSI, C; STAMATOPOULOS, K, 2014:IgG-Switched CLL Has a Distinct Immunogenetic Signature from the Common MD Variant: Ontogenetic Implications. CLINICAL CANCER RESEARCH 20 (2), p. 323 - 330.
• VERNER, J; TRBUSEK, M; CHOVANCOVA, J; SKABRAHOVA, H; JASKOVA, Z; MOULIS, M; FOLBER, F; HALOUZKA, R; MAYER, J; POSPISILOVA, S; DOUBEK, M, 2014:NOD/SCID GAMMA XENOGRAFT MODEL OF B-CELL MALIGNANCIES USING TP53-AND/OR ATM-DEFICIENT CELL LINES. HAEMATOLOGICA 99 , p. 312 - 313.
• XOCHELLI, A; AGATHANGELIDIS, A; KAVAKIOTIS, I; MINGA, E; SUTTON, LA; BALIAKAS, P; CHOUVARDA, I; GIUDICELLI, V; VLAHAVAS, I; MAGLAVERAS, N; BONELLO, L; TRENTIN, L; MONTILLO, M; PANAGIOTIDIS, P; GEISLER, C; LANGERAK, AW; POSPISILOVA, S; JELINEK, DF; OSCIER,, 2014:IMMUNOGLOBULIN HEAVY VARIABLE GENES AND ALLELES: NEW ENTITIES, NEW NAMES AND IMPLICATIONS FOR RESEARCH AND PROGNOSTICATION IN CLL. HAEMATOLOGICA 99 , p. 49 - 49.
• XOCHELLI, A; KAVAKIOTIS, I; AGATHANGELIDIS, A; KIENLE, D; DAVIS, Z; YAN, XJ; SHANAFELT, T; BOUDJOGRA, M; PLEVOVA, K; CHATZOULI, M; PEDERSEN, LB; VERONESE, S; FACCO, M; MORENO, D; CHU, CC; GIUDICELLI, V; PANAGIOTIDIS, P; VLAHAVAS, I; ANAGNOSTOPOULOS, A; MAGLAVERAS, N; TRENTIN, L; CATHERWOOD, M; MONTILLO, M; GEISLER, CH; LANGERAK, AW; POSPISILOVA, S; LEFRANC, MP; CHIORAZZI, N; OSCIER, D; JELINEK, DF; STILGENBAUER, S; BELESSI, C; GHIA, P; DAVI, F; ROSENQUIST, R; DARZENTAS, N; CHOUVARDA, I; SUTTON, LA; HADZIDIMITRIOU, A; STAMATOPOULOS, K, 2014:Charting Unique Signatures of Somatic Hypermutation Amongst Chronic Lymphocytic Leukemia Patients Expressing IGHV4-34 Clonotypic B Cell Receptors. BLOOD 124 (21)

2013

• BACCARANI, M; DEININGER, MW; ROSTI, G; HOCHHAUS, A; SOVERINI, S; APPERLEY, JF; CERVANTES, F; CLARK, RE; CORTES, JE; GUILHOT, F; HJORTH-HANSEN, H; HUGHES, TP; KANTARJIAN, HM; KIM, DW; LARSON, RA; LIPTON, JH; MAHON, FX; MARTINELLI, G; MAYER, J; MULLER, MC; , 2013:European LeukemiaNet recommendations for the management of chronic myeloid leukemia: 2013. BLOOD 122 (6), p. 872 - 884.
• FINK, AM; BOTTCHER, S; RITGEN, M; FISCHER, K; PFLUG, N; EICHHORST, B; WENDTNER, CM; WINKLER, D; BUHLER, A; ZENZ, T; STAIB, P; MAYER, J; HENSEL, M; HOPFINGER, G; WENGER, M; FINGERLE-ROWSON, G; DOHNER, H; KNEBA, M; STILGENBAUER, S; BUSCH, R; HALLEK, M, 2013:Prediction of poor outcome in CLL patients following first-line treatment with fludarabine, cyclophosphamide and rituximab. LEUKEMIA 27 (9), p. 1949 - 1952.
• HOFFMANN, VS; BACCARANI, M; LINDOERFER, D; CASTAGNETTI, F; TURKINA, A; ZARITSKY, A; HELLMANN, A; PREJZNER, W; STEEGMANN, JL; MAYER, J; INDRAK, K; COLITA, A; ROSTI, G; PFIRRMANN, M, 2013:The EUTOS prognostic score: review and validation in 1288 patients with CML treated frontline with imatinib. LEUKEMIA 27 (10), p. 2016 - 2022.
• KAUCKA, M; PLEVOVA, K; PAVLOVA, S; JANOVSKA, P; MISHRA, A; VERNER, J; PROCHAZKOVA, J; KREJCI, P; KOTASKOVA, J; OVESNA, P; TICHY, B; BRYCHTOVA, Y; DOUBEK, M; KOZUBIK, A; MAYER, J; POSPISILOVA, S; BRYJA, V, 2013:The Planar Cell Polarity Pathway Drives Pathogenesis of Chronic Lymphocytic Leukemia by the Regulation of B-Lymphocyte Migration. CANCER RESEARCH 73 (5), p. 1491 - 1501.
• KHARE, V; LYAKHOVICH, A; DAMMANN, K; LANG, M; BORGMANN, M; TICHY, B; POSPISILOVA, S; LUCIANI, G; CAMPREGHER, C; EVSTATIEV, R; PFLUEGER, M; HUNDSBERGER, H; GASCHE, C, 2013:Mesalamine modulates intercellular adhesion through inhibition of p-21 activated kinase-1. BIOCHEMICAL PHARMACOLOGY 85 (2), p. 234 - 244.
• KLAMPFL, T; MILOSEVIC, JD; PUDA, A; SCHONEGGER, A; BAGIENSKI, K; BERG, T; HARUTYUNYAN, AS; GISSLINGER, B; RUMI, E; MALCOVATI, L; PIETRA, D; ELENA, C; DELLA PORTA, MG; PIERI, L; GUGLIELMELLI, P; BOCK, C; DOUBEK, M; DVORAKOVA, D; SUVAJDZIC, N; TOMIN, D; TOS, 2013:Complex Patterns of Chromosome 11 Aberrations in Myeloid Malignancies Target CBL, MLL, DDB1 and LMO2. PLOS ONE 8 (10)
• KREJCI, M; DOUBEK, M; BRYCHTOVA, Y; STEHLIKOVA, O; CHOVANCOVA, J; TICHY, B; FRANCOVA, HS; NAVRATIL, M; TOMISKA, M; HORKY, O; POSPISILOVA, S; MAYER, J, 2013:Fludarabine with cytarabine followed by reduced-intensity conditioning and allogeneic hematopoietic stem cell transplantation in patients with poor-risk chronic lymphocytic leukemia. ANNALS OF HEMATOLOGY 92 (2), p. 249 - 254.
• PAGANO, L; CORNELY, OA; BUSCA, A; CAIRA, M; CESARO, S; GASBARRINO, C; GIRMENIA, C; HEINZ, WJ; HERBRECHT, R; LASS-FLORL, C; NOSARI, A; POTENZA, L; RACIL, Z; RICKERTS, V; SHEPPARD, DC; SIMON, A; ULLMANN, AJ; VALENTINI, CG; VEHRESCHILD, JJ; CANDONI, A; VEHRE, 2013:Combined antifungal approach for the treatment of invasive mucormycosis in patients with hematologic diseases: a report from the SEIFEM and FUNGISCOPE registries. HAEMATOLOGICA 98 (10)
• POTLUKOVA, E; FREIBERGER, T; LIMANOVA, Z; JISKRA, J; TELICKA, Z; BARTAKOVA, J; SPRINGER, D; VITKOVA, H; TRENDELENBURG, M, 2013:Association between Low Levels of Mannan-Binding Lectin and Markers of Autoimmune Thyroid Disease in Pregnancy. PLOS ONE 8 (12)
• RACIL, Z; RAZGA, F; DRAPALOVA, J; BURESOVA, L; ZACKOVA, D; PALACKOVA, M; SEMERAD, L; MALASKOVA, L; HALUZIK, M; MAYER, J, 2013:Mechanism of impaired glucose metabolism during nilotinib therapy in patients with chronic myelogenous leukemia. HAEMATOLOGICA 98 (10)
• REBLOVA, M; UNTEREINER, WA; REBLOVA, K, 2013:Novel Evolutionary Lineages Revealed in the Chaetothyriales (Fungi) Based on Multigene Phylogenetic Analyses and Comparison of ITS Secondary Structure. PLOS ONE 8 (5)
• SEDO, O; NEMEC, A; KRIZOVA, L; KACALOVA, M; ZDRAHAL, Z, 2013:Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. SYSTEMATIC AND APPLIED MICROBIOLOGY 36 (8), p. 572 - 578.
• SKALOVA, D; ZIDKOVA, J; VOHANKA, S; MAZANEC, R; MUSOVA, Z; VONDRACEK, P; MRAZOVA, L; KRAUS, J; REBLOVA, K; FAJKUSOVA, L, 2013:CLCN1 Mutations in Czech Patients with Myotonia Congenita, In Silico Analysis of Novel and Known Mutations in the Human Dimeric Skeletal Muscle Chloride Channel. PLOS ONE 8 (12)
• STANDARA, S; KULHANEK, P; MAREK, R; STRAKA, M, 2013:Xe-129 NMR chemical shift in Xe@C-60 calculated at experimental conditions: Essential role of the relativity, dynamics, and explicit solvent. JOURNAL OF COMPUTATIONAL CHEMISTRY 34 (22), p. 1890 - 1898.
• SUTTON, LA; AGATHANGELIDIS, A; BELESSI, C; DARZENTAS, N; DAVI, F; GHIA, P; ROSENQUIST, R; STAMATOPOULOS, K, 2013:Antigen selection in B-cell lymphomas-Tracing the evidence. SEMINARS IN CANCER BIOLOGY 23 (6), p. 399 - 409.
• SUTTON, LA; KOSTARELI, E; STALIKA, E; TSAFTARIS, A; ANAGNOSTOPOULOS, A; DARZENTAS, N; ROSENQUIST, R; STAMATOPOULOS, K, 2013:Temporal Dynamics of Clonal Evolution in Chronic Lymphocytic Leukemia with Stereotyped IGHV4-34/IGKV2-30 Antigen Receptors: Longitudinal Immunogenetic Evidence. MOLECULAR MEDICINE 19 , p. 230 - 236.
• VYCHODILOVA, L; MATIASOVIC, J; BOBROVA, O; FUTAS, J; KLUMPLEROVA, M; STEJSKALOVA, K; CVANOVA, M; JANOVA, E; OSICKOVA, J; VYSKOCIL, M; SEDLINSKA, M; DUSEK, L; MARTI, E; HORIN, P, 2013:Immunogenomic analysis of insect bite hypersensitivity in a model horse population. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 152 (42097), p. 260 - 268.
• ZD'ARSKA, M; ZATLOUKALOVA, P; BENITEZ, M; SEDO, O; POTESIL, D; NOVAK, O; SVACINOVA, J; PESEK, B; MALBECK, J; VASICKOVA, J; ZDRAHAL, Z; HEJATKO, J, 2013:Proteome Analysis in Arabidopsis Reveals Shoot- and Root-Specific Targets of Cytokinin Action and Differential Regulation of Hormonal Homeostasis. PLANT PHYSIOLOGY 161 (2), p. 918 - 930.

2012

• AGATHANGELIDIS, A; DARZENTAS, N; HADZIDIMITRIOU, A; BROCHET, X; MURRAY, F; YAN, XJ; DAVIS, Z; VAN GASTEL-MOL, EJ; TRESOLDI, C; CHU, CC; CAHILL, N; GIUDICELLI, V; TICHY, B; PEDERSEN, LB; FORONI, L; BONELLO, L; JANUS, A; SMEDBY, K; ANAGNOSTOPOULOS, A; MERLE, 2012:Stereotyped B-cell receptors in one-third of chronic lymphocytic leukemia: a molecular classification with implications for targeted therapies. BLOOD 119 (19), p. 4467 - 4475.
• BIKOS, V; STALIKA, E; BALIAKAS, P; DARZENTAS, N; DAVIS, Z; TRAVERSE-GLEHEN, A; DAGKLIS, A; KANELLIS, G; ANAGNOSTOPOULOS, A; TSAFTARIS, A; PONZONI, M; BERGER, F; FELMAN, P; GHIA, P; PAPADAKI, T; OSCIER, D; BELESSI, C; STAMATOPOULOS, K, 2012:Selection of antigen receptors in splenic marginal-zone lymphoma: further support from the analysis of the immunoglobulin light-chain gene repertoire. LEUKEMIA 26 (12), p. 2567 - 2569.
• BIKOS, V; STALIKA, E; KARYPIDOU, M; BALIAKAS, P; DAVIS, Z; ALGARA, P; KANELLIS, G; DAGKLIS, A; TRAVERSE-GLEHEN, A; HOCKLEY, S; MOLLEJO, M; TSAFTARIS, A; ANAGNOSTOPOULOS, A; PONZONI, M; BERGER, F; FELMAN, P; GONZALEZ, D; MATUTES, E; PIRIS, MA; PAPADAKI, T; GHIA, P; OSCIER, D; BELESSI, C; DARZENTAS, N; STAMATOPOULOS, K, 2012:Clonal Selection in the Ontogeny and Evolution of Splenic Marginal Zone Lymphoma Confirming the Existence of Distinct Molecular Subtypes. BLOOD 120 (21)
• BOUSIOS, A; MINGA, E; KALITSOU, N; PANTERMALI, M; TSABALLA, A; DARZENTAS, N, 2012:MASiVEdb: the Sirevirus Plant Retrotransposon Database. BMC GENOMICS 13
• CINCAROVA, L; LOCHMANOVA, G; NOVAKOVA, K; SULTESOVA, P; KONECNA, H; FAJKUSOVA, L; FAJKUS, J; ZDRAHAL, Z, 2012:A combined approach for the study of histone deacetylase inhibitors. MOLECULAR BIOSYSTEMS 8 (11), p. 2937 - 2945.
• CORNELISSEN, JJ; GRATWOHL, A; SCHLENK, RF; SIERRA, J; BORNHAUSER, M; JULIUSSON, G; RACIL, Z; ROWE, JM; RUSSELL, N; MOHTY, M; LOWENBERG, B; SOCIE, G; NIEDERWIESER, D; OSSENKOPPELE, GJ, 2012:The European LeukemiaNet AML Working Party consensus statement on allogeneic HSCT for patients with AML in remission: an integrated-risk adapted approach. NATURE REVIEWS CLINICAL ONCOLOGY 9 (10), p. 579 - 590.
• DOLEZALOVA, D; MRAZ, M; BARTA, T; PLEVOVA, K; VINARSKY, V; HOLUBCOVA, Z; JAROS, J; DVORAK, P; POSPISILOVA, S; HAMPL, A, 2012:MicroRNAs Regulate p21(Waf1/Cip1) Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells. STEM CELLS 30 (7), p. 1362 - 1372.
• GANOPOULOS, I; MADESIS, P; DARZENTAS, N; ARGIRIOU, A; TSAFTARIS, A, 2012:Barcode High Resolution Melting (Bar-HRM) analysis for detection and quantification of PDO "Fava Santorinis" (Lathyrus clymenum) adulterants. FOOD CHEMISTRY 133 (2), p. 505 - 512.
• JANIKOVA, A; MARECKOVA, A; DVORAKOVA, D; BORTLICEK, Z; TICHY, B; NAVRATIL, M; KRAL, Z; POSPISILOVA, S; MAYER, J, 2012:A real-time (PCR) for a real life ...? Quantitative evaluation of BCL2/IGH in follicular lymphoma and its implications for clinical practice. EXPERIMENTAL HEMATOLOGY 40 (7), p. 528 - 539.
• KOSTARELI, E; GOUNARI, M; JANUS, A; MURRAY, F; BROCHET, X; GIUDICELLI, V; POSPISILOVA, S; OSCIER, D; FORONI, L; DI CELLE, PF; TICHY, B; PEDERSEN, LB; JURLANDER, J; PONZONI, M; KOUVATSI, A; ANAGNOSTOPOULOS, A; THOMPSON, K; DARZENTAS, N; LEFRANC, MP; BELESS, 2012:Antigen receptor stereotypy across B-cell lymphoproliferations: the case of IGHV4-59/IGKV3-20 receptors with rheumatoid factor activity. LEUKEMIA 26 (5), p. 1127 - 1131.
• KOZUBIK, KS; MALCIKOVA, J; TRBUSEK, M; TOM, N; DOUBEK, M; BRYCHTOVA, Y; SMARDOVA, J; MAYER, J; POSPISILOVA, S; TICHY, B, 2012:Clonal Selection of TP53 Mutations in Chronic Lymphocytic Leukaemia Detected by Ultra-deep Pyrosequencing. EUROPEAN JOURNAL OF CANCER 48 , p. S146 - S146.
• LENGEROVA, M; KOCMANOVA, I; RACIL, Z; HRNCIROVA, K; POSPISILOVA, S; MAYER, J; NAJVAR, LK; WIEDERHOLD, NP; KIRKPATRICK, WR; PATTERSON, TF, 2012:Detection and Measurement of Fungal Burden in a Guinea Pig Model of Invasive Pulmonary Aspergillosis by Novel Quantitative Nested Real-Time PCR Compared with Galactomannan and (1,3)-beta-D-Glucan Detection. JOURNAL OF CLINICAL MICROBIOLOGY 50 (3), p. 602 - 608.
• MRAZ, M; DOLEZALOVA, D; PLEVOVA, K; KOZUBIK, KS; MAYEROVA, V; CERNA, K; MUSILOVA, K; TICHY, B; PAVLOVA, S; BORSKY, M; VERNER, J; DOUBEK, M; BRYCHTOVA, Y; TRBUSEK, M; HAMPL, A; MAYER, J; POSPISILOVA, S, 2012:MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia. BLOOD 119 (9), p. 2110 - 2113.
• MRAZ, M; POSPISILOVA, S, 2012:MicroRNAs in chronic lymphocytic leukemia: from causality to associations and back. EXPERT REVIEW OF HEMATOLOGY 5 (6), p. 579 - 581.

2011

• JANIKOVA, A; TICHY, B; SUPIKOVA, J; STANO-KOZUBIK, K; POSPISILOVA, S; KREN, L; VASOVA, I; SALEK, D; MAYER, J, 2011:Gene expression profiling in follicular lymphoma and its implication for clinical practice. LEUKEMIA & LYMPHOMA 52 (1), p. 59 - 68.
• MRAZ, M; ZENT, CS; CHURCH, AK; JELINEK, DF; WU, XS; POSPISILOVA, S; ANSELL, SM; NOVAK, AJ; KAY, NE; WITZIG, TE; NOWAKOWSKI, GS, 2011:Bone marrow stromal cells protect lymphoma B-cells from rituximab-induced apoptosis and targeting integrin alpha-4-beta-1 (VLA-4) with natalizumab can overcome this resistance. BRITISH JOURNAL OF HAEMATOLOGY 155 (1), p. 53 - 64.
• TRBUSEK, M; SMARDOVA, J; MALCIKOVA, J; SEBEJOVA, L; DOBES, P; SVITAKOVA, M; VRANOVA, V; MRAZ, M; FRANCOVA, HS; DOUBEK, M; BRYCHTOVA, Y; KUGLIK, P; POSPISILOVA, S; MAYER, J, 2011:Missense Mutations Located in Structural p53 DNA-Binding Motifs Are Associated With Extremely Poor Survival in Chronic Lymphocytic Leukemia. JOURNAL OF CLINICAL ONCOLOGY 29 (19), p. 2703 - 2708.
• VAN DER FITS, L; SANDBERG, Y; DARZENTAS, N; ZOUTMAN, WH; TIELEMANS, D; WOLVERS-TETTERO, ILM; VERMEER, MH; LANGERAK, AW, 2011:A restricted clonal T-cell receptor alpha beta repertoire in Sezary syndrome is indicative of superantigenic stimulation. BRITISH JOURNAL OF DERMATOLOGY 165 (1), p. 78 - 84.
• VARGOVA, K; CURIK, N; BURDA, P; BASOVA, P; KULVAIT, V; POSPISIL, V; SAVVULIDI, F; KOKAVEC, J; NECAS, E; BERKOVA, A; OBRTLIKOVA, P; KARBAN, J; MRAZ, M; POSPISILOVA, S; MAYER, J; TRNENY, M; ZAVADIL, J; STOPKA, T, 2011:MYB transcriptionally regulates the miR-155 host gene in chronic lymphocytic leukemia. BLOOD 117 (14), p. 3816 - 3825.
• WALEWSKI, J; PASZKIEWICZ-KOZIK, E; WARSZEWSKA, A; BORSARU, G; MOICEAN, A; HELLMANN, A; MAYER, J; HAUNS, B; MAIS, A; HENNING, SW; HENTSCH, B, 2011:Final Results of the Phase II SAPHIRE Trial of Resminostat (4SC-201) in Patients with Relapsed/Refractory Hodgkin Lymphoma. BLOOD 118 (21), p. 1149 - 1149.

2010

• DARZENTAS, N; HADZIDIMITRIOU, A; MURRAY, F; HATZI, K; JOSEFSSON, P; LAOUTARIS, N; MORENO, C; ANAGNOSTOPOULOS, A; JURLANDER, J; TSAFTARIS, A; CHIORAZZI, N; BELESSI, C; GHIA, P; ROSENQUIST, R; DAVI, F; STAMATOPOULOS, K, 2010:A different ontogenesis for chronic lymphocytic leukemia cases carrying stereotyped antigen receptors: molecular and computational evidence. LEUKEMIA 24 (1), p. 125 - 132.
• KOTASKOVA, JANA; TICHY, BORIS; TRBUSEK, MARTIN; FRANCOVA, HANA SKUHROVA; KABATHOVA, JITKA; MALCIKOVA, JITKA; DOUBEK, MICHAEL; BRYCHTOVA, YVONA; MAYER, JIRI; POSPISILOVA, SARKA, 2010:High Expression of Lymphocyte-Activation Gene 3 (LAG3) in Chronic Lymphocytic Leukemia Cells Is Associated with Unmutated lmmunoglobulin Variable Heavy Chain Region (IGHV) Gene and Reduced Treatment-Free Survival. JOURNAL OF MOLECULAR DIAGNOSTICS 12 (3), p. 328 - 334.
• MALCIKOVA, J; TICHY, B; DAMBORSKY, J; KABATHOVA, J; TRBUSEK, M; MAYER, J; POSPISILOVA, S, 2010:Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation. BIOLOGICAL CHEMISTRY 391 (2 III), p. 197 - 205.

2009

• MRAZ, M., MALINOVA, K., KOTASKOVA, J., PAVLOVA, S., TICHY, B., MALCIKOVA, J., STANO-KOZUBIK, K., SMARDOVA, J., BRYCHTOVA, Y., DOUBEK, M., TRBUSEK, M., MAYER, J., POSPISILOVA, S., 2009:miR-34a, miR-29c and miR-17-5p are downregulated in CLL patients with TP53 abnormalities. Leukemia 23 (6), p. 1159 - 1163.

GRANTY

• Expression and function of activation-induced cytidine deaminase splice variants in normal B cell physiology and chronic lymphocytic leukemia (3SGA5792), South Moravian Region - SoMoPro, 2013 - 2016
• Analysis of changes in genome and its expression in chronic lymphocytic leukemia patients during the disease development (NS10439), Ministry of Health - Departmental Programme of Research and Development (2008-2011), 2009 - 2011
• Funkční a strukturní změny microRNA u lymfoproliferativních malignit a jejich vliv na prognózu onemocnění a predikci léčebné odpovědi (NT11218), Ministry of Health - Ministry of Health's Departmental Research and Development Programme III (2010-2015), 2010 - 2015
• Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie (MSM0021622430), MEYS - Institutional Research Plan, 2007 - 2013
• Real-time PCR soupravy pro diagnostiku v onkologii (FR-TI2/254), Ministry of Industry and Trade - TIP, 2010 - 2013
• Podpora profesního růstu a mezinárodní integrace výzkumných týmů v oblasti molekulární medicíny (CZ.1.07/2.3.00/20.0045), MEYS - OP Education for Competiteveness, 2011 - 2014
• Involvement of nuclear HMGB proteins in sensitizing of human cells to anticancer drugs inhibiting DNA topoizomerases (GAP301/10/0590), Czech Science Foundation - Standard Grants, 2010 - 2012

CURRENT RESEARCH INFRASTRUCTURE

Molecular – biological diagnostic and research laboratory equipped with instrumentation for routine diagnostic tasks such as PCR, qPCR, DNA electrophoresis and DNA sequencing as well as equipment serving mainly for research needs such as microarray scanners (short and long oligonucleotide platforms) or nucleofector for high-efficient transfections. 

1. Analysis of genomic data in patients with chronic lymphocytic leukemia

Supervisor: Mgr. Karla Plevová, Ph.D.
Consultants: Mgr. Kamila Réblová, Ph.D., Mgr. Karol Pál

Annotation:

With the advent of massively parallel sequencing (MPS), many challenges in molecular biology and medicine have emerged including the discovery of novel molecular mechanisms and biological networks. Due to the accelerated development in genomics and computational technologies it is possible to study molecular processes at various levels ranging from primary DNA structure, through genome organization, to its expression in a comprehensive manner. Among other biological contexts this is especially important in human diseases, such as cancer. In the proposed PhD project, a PhD candidate will perform advanced bioinformatics analyses of genomic data obtained using MPS in cases with chronic lymphocytic leukemia (CLL). The student will focus on discovery of genetic and expression patterns related to specific disease conditions, namely CLL early development, and accumulation of adverse genomic defects leading to refractory disease. In particular, tumor signatures will be extracted from DNA and RNA sequencing data in an attempt to provide a detailed picture of molecular processes underlying CLL clonal evolution. It is expected that the student will acquire good knowledge of existing solutions, design his/her own bioinformatics tools and employ computing approaches based on machine learning, if necessary. Obtained data will be validated using available independent datasets and will serve as a basis for consequent wet-lab experiments aiming to define novel clinically relevant biomarkers for better disease stratification and management.

Keywords: chronic lymphocytic leukemia, cancer genome, clonal evolution, biomarkers, mutation signature, expression pattern, tumor interactions, bioinformatics tools, computing technologies