Inverted microscope Zeiss Axio Observer.Z1 with confocal unit LSM 800. Microscope is equipped with four solid state lasers - 405, 488, 561, 640nm. For detection of fluorescent light in confocal mode there are three GaAsP PMT detectors and T-PMT detector for transmission light detection.For acquisition in widefield mode there is AxioCam 503 CCD camera.
Lasers: 405, 488, 561, 640 nm.
Detectors: 3 x GaAsp PMT, 1 x T- PMT detector.
Filters for fluorescence: DAPI, GFP, Cy3, Cy5.
Camera: Axiocam 503, CCD sensor, 1936 x 1460 pixels, pixel size 4.54 x 4.54 um.
Objectives: 10x/0.3 – EC Plan-Neofluar; 25x/0.8 – LCI Plan-Neofluar; 40x/1.3 – Plan-Apochromat; 63x/1.2 – C-Apochromat; 63x/1.4 – Plan-Apochromat.
Software: ZEN blue.
