Cost-effective spinning disc microscopy – hands-on workshop

Nikon-CrEST Spinning disk system

Key specifications and features

• Resolution: 100X Plan Apo Lambda 1.45 objective and 70 μm pinhole: 250 nm lateral; 650 nm axial
• Disk Speed: 15.000 rpm standard, 20.000 rpm version possible
• Illumination: LED – Spectra X (Lumencor) or CAIRN Laser unit
• Single or dual pattern option, up to 22mm FOV (single pattern)
• Pattern pinhole size: 40 μm (for low NA objectives) and/or 70 μm (for high NA objectives). Custom size and pattern on request
• Ease of use: NIS Elements driven interface. Easy pattern focusing
• Objective compatibility: 100X, 60X, 40X
• Possibility for: VCS hardware resolution enhancement or deconvolution software resolution enhancement with NIS Elements software

Technology

• CrEST spinning disk uses a proprietary NA dependent pinhole pattern for maximum confocality and higher S/N ratios
• Single pattern (1 pinhole size, large FOV) or dual pattern (2 pinhole sizes, but smaller FOV)
• Motorized widefield to confocal switchover while spinning
• Easy Gimbal mount for quick alignment and best S/N
• Continuous spiral pattern available for higher throughput
• Motorized dichroic and emitter filter wheels

Registration is CLOSED