Confocal microscopy enables high resolution imaging of fluorescence labelled objects. High resolution is achieved by scaning fluorescence exclusively from a focused plane. This is achieved by using lasers as a source of excitation light and photomultiplier coupled with a pinhole, which filters out the emission ligh comming from unfocused planes. Confocal laser-scanning mikroscopy produces series of optical slices through the sample that are reconstructed to a complete high-resolution image using a specialized software.
Research group:
Functional Genomics and Proteomics of Plants - Jan Hejátko
